Issue 1 (197)/2024

Cover Story

Jellyfish (JF) are essential to marine ecosystems. However, JF that develop rapidly can have negative effects. On 3–4 August, 2022, a significant JF (Lobonemoides robustus Stiasny, 1920) bloom was observed along the Cox’s Bazar coast in Bangladesh (from Najdirartek to Sabrang). The goal of the current investigation was to identify the fatty acids (FAs) and amino acids (AAs) of L. robustus. The AAs were determined using liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis, while the FAs were determined using a gas chromatographic system with a flame ionisation detector. The most prevalent AA was glycine. The most common FA was linoleic acid (C18:3) (0.43%), followed by myristic acid (0.12%), cis-9-oleic acid (0.18%), gamma-linolenic acid (0.24%), and heptadecanoic acid (0.29%). Based on its high AA and FA contents, L. robustus is a great candidate for the potentially sustainable manufacture of nutraceutical, cosmeceutical, and biomedical natural products to improve health and well-being. In addition, the edible L. robustus could be exported to other countries for use as the bloom species, thus playing a major role in achieving a blue economy (see page 107).

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Extraction and Characterisation of Natural Dye From Orange Peel for Textile Applications

Usman Habu Taura, Muhammad Abbagoni Abubakar, Abdulhalim Musa Abubakar, Mohammed Umar Kurgiya

ABSTRACT. Awareness of the need to protect the environment and people’s health has led to an intensification of concerns for obtaining sustainable products and processes. Toxic waste created during the production and use of synthetic dyes has an impact on both human and environmental health. As a result, natural dyes are more secure and safer than synthetic dyes. This study is significant because it has the potential to help develop sustainable and environmentally friendly textile dyeing techniques. In this study, a natural dye was successfully extracted from orange peel (Citrus recticulata Rutaceae) which was applied on textile fibre and was found to be partly effective for eco-friendly dyeing applications. The extracted dye does not have a good wash fastness, which is the ability of the fabric to retain its colour after washing. This weakness is demonstrated by carrying out a Fourier transform infrared (FTIR) analysis where the potential binding mechanisms between the dye and the textile fibres was revealed. Different functional groups can interact with the fibre’s functional groups, affecting the dye’s affinity for the fabric and its overall colour fastness properties. Findings show that dyes from orange peels showed promise in this study, but need to be improved further. Therefore, the study suggests that further research is needed to optimize the dyeing process and improve the fabric’s resistance to washing and other environmental factors.

Keywords: mordant; natural dye; orange peel; synthetic dye; textile industry.

Correction: Usman Habu TAURA’s affiliation has been changed from Department of Chemical Engineering, University of Maiduguri, P. M.B 1069 Maiduguri, Borno State, Nigeria in Oil and Gas Research Centre, Sultan Qaboos University, Muscat, Oman, 123, according to supporting document Journal of Applied Life Sciences and Environment_affiliation correction justification

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Assessment of Sensitivity to Desertification in the Oum Er-Rbia Watershed (Upstream of Ouled Sidi Driss) Using The Medalus Approach

Souad Ourabit, Abderrahim Ettaqy, Mohamed El Ghachi

ABSTRACT. The issue of desertification is a pressing concern for many vulnerable regions, with consequences that extend far beyond their borders. It is a silent force that not only contributes to global climate change by releasing stored carbon from vegetation and soil but also compounds the ongoing environmental challenges we face on a global scale. In Morocco, the effects of these climate changes are already noticeable, particularly in terms of water scarcity due to reduced rainfall and rising temperatures. This, in turn, leads to soil drying and an increased risk of degradation. The Oum Er-Rbia watershed (upstream of Ouled Sidi Driss) is one of the basins affected by this issue, covering an area of 11,152 km² and spanning three topographic units (the Middle Atlas Mountains, the Phosphate Plateau and the Tadla Plain). This topographic diversity causes regional variation in the sensitivity to desertification. Therefore, this study aims to evaluate the sensitivity to desertification in this watershed by using the MEDALUS model (Mediterranean desertification and land use), which calculates the Desertification Sensitivity Index (DSI) through the composition of four indices: the Soil Quality Index (SQI), the Vegetation Quality Index (VQI), the Climate Quality Index (CQI) and the Land Use Quality Index (LUQI). The results show that the critical and highly sensitive zones represent 44% and are located at the northeast and west ends of the watershed. By contrast, the unaffected zones represent 12% of the total area of the watershed and correspond to the upstream area, where the climate, vegetation and land use systems are of good quality, providing effective protection for the land. To reduce the impact of this phenomenon, we have proposed some potentially impactful development actions based on land use and the results obtained.

Keywords: climate change; desertification sensitivity; drought; MEDALUS; Oum Er-Rbia watershed.

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Growth Performance, Nitrogen Utilization and Economics of Red Sokoto Bucks Fed Saccharomyces Cerevisiae-Treated Groundnut Haulm and Dried Cassava Peel-Based Diets

Ahmad Rufai Musa, Olayinka John Makinde, Aminu Maidala, Ahmed Bishir, Kakudi Ismail Abubakar, Kosoro Ahmad Mua’zu, Ismaila Habiba Abdullahi, Yakubu Zakari Idrissa, Charles Hannatu, Muhammad Hamisu Zango

ABSTRACT. This study investigated the growth performance, nitrogen balance, and economics of production of Red Sokoto bucks (RSBs) fed Saccharomyces cerevisiae-treated, biodegradable groundnut haulms (SCGH) and dried cassava peels (DCP). Four dietary treatments were formulated including a control diet (T1; 0% SCGH:DCP) and DCP in combination with SCGH at the different proportions of 275:695 (T2), 375:595 (T3), and 475:495 (T4) g/kg of dry matter (DM). A combination of 5 g and 50 g of yeast as a probiotic and molasses, respectively, were added to 1 kg of GH after been dissolved in 1 L of water. Twelve healthy RSBs weighing 9.5 ± 0.40 kg of body weight (BW) and aged 7 to 8 months were exposed to four treatments. This was replicated three times in a completely randomized design (CRD). The experiment lasted for 90 days. The average final weight, average daily weight gain (gram/day), nitrogen intake, and nitrogen balance were significantly influenced by the inclusion of DCP and SCGH in the diets. There was no significant difference (p > 0.05) between the ADG of T1 (58.20 g/d) and the T4 (58.89 g/d) groups. Feed conversion ratio and feed-cost saving ($630.80) increased with the inclusion of DCP and SCGH in the goats’ diets. In conclusion, T4 (SCGH: 475g, DCP: 495g) had a better chemical composition, ADG, nitrogen balance, and feed-cost saving; therefore, SCGH are recommended and are readily available agricultural wastes that can be fed to RSBs to improve performance and nitrogen utilization in a cost-effective way, especially during dry season.

Keywords: bucks; cassava peels; nitrogen balance; probiotics; yeast.

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First Report of The Amino Acid And Fatty Acid Composition of Jellyfish (Lobonemoides Robustus Stiasny, 1920) Collected During Jellyfish Bloom Along the Cox’s Bazar Coast, Bangladesh

Tarikul Islam, Simul Bhuyan, Mala Khan, Mrityunjoy Kunda, Sumi Akter, Nayan Kumer Kundu

ABSTRACT. Jellyfish (JF) are essential to marine ecosystems. However, JF that increases rapidly can have negative effects. On 3-4 August 2022, a significant JF (Lobonemoides robustus Stiasny, 1920) bloom was observed along Cox’s Bazar coast (from Najdirartek to Sabrang) in Bangladesh. The goal of the current investigation was to identify the fatty acids (FAs) and amino acids (AAs) of L. robustus. The AAs were determined using liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis, while the FAs were determined using a gas chromatographic system with a flame ionisation detector. The most prevalent AA was glycine. The most common FA was linoleic acid (C18:3) (0.43%), followed by myristic acid (0.12%), cis-9-oleic acid (0.18%), gamma-linolenic acid (0.24%), and heptadecanoic acid (0.29%). Based on its AA and FA contents, L. robustus can be a great candidate for the potentially sustainable manufacture of nutraceutical, cosmeceutical, and biomedical natural products to improve health and well-being. In addition, the edible L. robustus could be exported to other countries, thus way it can play a major role in achieving a blue economy.

Keywords: amino acids; bloom; blue economy; fatty acids; jellyfish.

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Foliar Zinc and Soil Applied Molybdenum Optimize Yield and Dry Matter Partitioning of Lentil

Shams Ur Rehman, Uzair Ahmed, Ayesha Zahoor, Zia Ur Rahman, Ikram Ullah, Salman Khan, Rizwan Ali, Osama Muhammad Jafar, Muhammad Taimoor, Aamir Sohail, Sajid Ali

ABSTRACT. A field experiment on the effect of zinc and molybdenum application on the yield of lentil was carried out at The University of Agriculture Peshawar during the Rabi season of 2021. The experiment was carried out in a randomised complete block design with three replications. Four levels of molybdenum (0, 1, 2 and 3 kg ha−1) and 0.5% zinc foliar spray at different intervals of lentil crop (control, 50 days after sowing, 100 days after sowing, 50 days after sowing + 100 days after sowing) were tested in the experiment. The results revealed that 3 kg ha−1 of molybdenum enhanced plant height (55 cm), nodules plant−1 (25), branches plant−1 (7), days to flowering (107) and maturity (159), dry matter of leaves at the flowering stage (64 g m−2), dry matter of leaves at the maturity stage (113 g m−2), dry matter of branches at the physiological maturity stage (304 g m−2), dry matter of pods at the maturity stage (439 g m−2), pods plant−1 (92), seeds pod−1 (2.0), 1000-seed weight (31 g), biological yield (3207 kg ha−1), and seed yield (1002 kg ha−1). Zinc foliar spray (0.5%) at 50 days after sowing + 100 days after sowing of lentil crop improved the dry matter of leaves at the flowering stage (62 g m−2), days to first flowering (109), days to physiological maturity (157), dry matter of leaves at the physiological maturity stage (111 g m−2), dry matter of pods at the physiological maturity stage (435 g m−2), pods plant−1 (91), 1000 seed weight (32 g), biological yield (3236 kg ha−1) and seed yield (1026 kg ha−1). Lastly, scatterplots revealed a significant correlation of grain and biological yield with their components. It is concluded that molybdenum application at a rate of 3 kg ha−1 and 0.5% zinc foliar spray at 50 days after sowing + 100 days after sowing of the lentil crop increased seed yield and is thus recommended for the Peshawar region.

Keywords: correlation; cropping system; fertilisation; Lens culinaris L.

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Estimation of Heritability, Genotypic Variability and Correlations Analysis for Yield and Yield Attributing Traits Among Bread Wheat (Triticum aestivum L.) Genotypes

Mansoor Javed, Akbar Ali, Muhammad Kashif, Muhammad Ali, Saif Ullah, Ayesha Alam

ABSTRACT. To find out genetic variability, heritability, and trait association among yield and yield-related traits among bread wheat genotypes, an alpha lattice design was used in triplicate manner where 50 wheat genotypes were evaluated at the University of Agriculture Peshawar during rabbi growing season 2021-22 along with a regional check genotype. Data were taken on ten parameters. All the genotypes showed significant variation among them, signifying the possibility of enhancing genetic improvement through breeding programs. Highly significant differences were found in days to heading, days to maturity, plant height, spike length, grain filling duration, number of grains per spike, biological yield and grain yield these traits indicating diversity in yield potential. Moderate to low heritability values were noted for most of the traits. The study exhibits positive correlations for plant height with grain yield, spike length with biological yield and harvest index with grain yield. On the basis of high heritability and positive correlation of grain yield with other traits, it is recommended that G-41, G-3, G-12, G-37, G-34 and G-14 genotypes which have the potential to be incorporate in further breeding programs.

Keywords: correlation; crop improvement; heritability; quantitative genetics; variability; wheat breeding; wheat genotypes.

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Preliminary Studies on Some Morpho-Structural and Biochemical Characterisation of Some Genotypes of Vitis vinifera L. Cultivated in Northeast Romania

Liliana Rotaru, Vasile Răzvan Filimon, Roxana Mihaela Filimon, Mihai Mustea, Roberto Renato Bernardis, Lucia Cintia Colibaba

ABSTRACT. The monitoring of new grapevine varieties with superior agrobiological and technological characteristics, in relation to the evolution of the climatic factors, represents an important and continuous objective of the worldwide viticultural research and breeding programs. Observations and determinations of the current study were performed on 11 new table grapes varieties created in Romania, growing in the Ampelographic collection of the University of Life Sciences Iasi, north-eastern area of Romania. The grapevine varieties were evaluated under the morpho-structural aspect, regarding the leaf area, the average weight of the grapes, the number and weight of the berries, rachis weight, the number and weight of the seeds, the weight of the skin and pulp, calculating the main technological indices. From a biochemical point of view, the content of photosynthetic pigments in leaves, the concentration of soluble dry solids and titratable acidity of the grapes were determined in the climatic condition of the Copou – Iasi vineyard. Therefore, the monitoring of genetic resources provides useful data for grape producers and researchers regarding the integration of new table grape varieties into the viticultural ecosystems, evaluating their yield and quality in correlation with the evolution and influence of the climatic factors.

Keywords: agrobiological and technological value; autochthonous grapevine variety; chemical composition; genetic resources; physico-structural characterization.

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Aspects of the Contribution of the Leader Approach to Rural Development in Romania. Case Study: North-East Development Region

Carmen-Olguța Brezuleanu, Mădălina-Maria Brezuleanu, Roxana Mihalache, Irina Susanu, Diana Elena Creangă, Elena Ungureanu

ABSTRACT. Rural development is the second pillar of the Common Agricultural Policy of the European Union (EU), with the role of helping the rural areas of the EU and implicitly Romania to address the economic, environmental, and social challenges they face. The purpose of the research included in this paper is to demonstrate the contribution of the LEADER approach to rural development in Romania, with an emphasis on the North-East Development Region. At the same time, the aim was to highlight the innovative nature of this approach: what it means, how it can be applied, and how it was applied. The data studied through the analysis carried out show that the Romanian territory and, implicitly, the North-East Development Region is poor, fragmented, depopulated, or in the process of depopulating, with few opportunities for young people. The innovative character of the LEADER Programme in Romania and implicitly the North-East Development Region results from the degree of novelty that an investment financed through it brings to the targeted territory, without being limiting and without necessarily presupposing a technological innovation, because the innovation must be evaluated relative to the local situation. The main instrument through which the principles of the LEADER approach can be implemented is the Local Action Group. It is the main driving force behind the activities to be carried out in the territory and which will lead to their implementation. The Local Action Groups set up in the North-East Region provide a common communication framework for local communities to develop and implement Local Development Strategies by initiating, developing and financing projects at local level. They contribute to the unity of local communities and their participation in local development. LEADER approach has brought and how its innovative character is highlighted. The questions that informed its development were: Is this concept considered as a model for sustainable rural development in Romania and the N-E Region? Is LEADER a truly innovative approach. In order to achieve the proposed goal, a multi-step working procedure was developed to allow the collection of target data and additional data derived from the initial target data. Thus, the working procedure was structured in the following steps: problem identification and conceptualization, literature review, document structuring, strategy selection, operational planning, data calculation, and interpretation. Both qualitative and quantitative methods were used in this work. Thus, from a quantitative point of view, the following research methods were considered relevant for obtaining data: administrative data analysis. As a qualitative method, a bibliometric analysis was carried out, i.e., the literature on sustainable rural development through the use of support measures was analysed by means of the VOSviewer programme, using the Web of Science collections database. Without the implementation of the LEADER Programme in Romania and implicitly in the North-East Development Region, rural areas may be deprived of funding that determines the improvement of conditions in that area, but efforts in the field of implementing sustainable rural development measures must be continued so that the effect of this funding is really visible. Thus, the results of the research carried out in the North-East Development Region of Romania add additional value to the information published in previous studies through proposals for rural reform and concrete examples of innovative projects implemented there.

Keywords: LEADER; local action group; North-East Development Region; Romania; rural development.

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Raw Bovine Milk as a Reservoir of Multi-Drug Resistant, Beta-Lactamase-Producing Klebsiella

Achintya Mahanti, Siddhartha Narayan Joardar, Samiran Bandyopadhyay, Jaydeep Banerjee, Sarbaswarup Ghosh, Tapan Kumar Dutta, Indranil Samanta

ABSTRACT. The transmission of zoonotic bacteria through consumption of raw milk is complicated by the dissemination of antimicrobial-resistant bacteria. The present study was conducted to detect the occurrence of antimicrobial-resistant bacteria (ESBL-/AmpC-producing Klebsiella spp.) in cow’s milk originating from healthy or infected (mastitis) cattle in India. In total, 450 milk samples were collected from apparently healthy cattle and cattle suffering from clinical or sub-clinical mastitis. Out of 455 Klebsiella spp., 67 (14.73%) isolates were found to be ESBL producers in the double-disc diffusion test. The occurrence of ESBL-producing Klebsiella spp. was significantly (p < 0.05) higher in milk samples collected from cattle suffering with mastitis than in healthy cattle. Among the ESBL-producing Klebsiella spp., 56 (83.6%) isolates were also detected that produced AmpC β-lactamases. All the ESBL and AmpC-producing Klebsiella spp. possessed blaCTX-M (100%) and blaAmpC (100%), respectively. The present study revealed a higher occurrence of class 1 integron in ESBL-producing Klebsiella spp. isolates. All ESBL-producing- Klebsiella spp. isolates were multi-drug resistant. The ciprofloxacin- and/or levofloxacin-resistant Klebsiella spp. isolates possessed the quinolone resistance gene (qnrS). The co-trimoxazole-resistant isolates possessed the sul1 and sul2 genes. Phylogenetic analysis of the studied isolates revealed that strains isolated from the same location had a clonal relationship. The study increases consumer awareness of the need to avoid raw milk consumption to prevent the spread of antimicrobial resistance in the community.
Keywords: antimicrobial resistance; ESBL; Klebsiella; MIC; raw milk.

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Physiological Particularities of Maize Plants and the Effect of Some Antioxidants Under Conditions of Moderate Drought

Anastasia Ștefîrță, Ion Bulhac, Lilia Brînză, Maria Cocu, Vera Zubareva

ABSTRACT. Complex investigation on the effect of Thiourea, Galmet and Thiogalmet compositions on water status, intensity of photosynthesis, water use efficiency, growth and yield of ‘P458’ maize plants under conditions of natural humidity in field trials was performed. The beneficial effect of seed and foliage pre-treatment with Thiourea, Galmet and, in particular, the new chemical composition Thiogalmet on plants’ biological processes conditioning a better realisation of the physiological processes associated with plant growth and productivity was established. A significantly greater positive impact of Thiogalmet on the optimisation of hydration degree, water retention capacity, stomatal conductance for CO2/H2O, assimilation, water use efficiency, plant growth and productivity was recorded. There was an additive action of Thiourea and Galmet in the composition of the Tiogalmet preparation. Thiogalmet increased the yield per unit area and improved the commercial quality of the grain. Treating plants with Thiourea, Galmet and Thiogalmet ensured a 27.20, 52.08 and 68.20% yield increase, respectively, compared to the plants in the control variant. Therefore, a major effect was registered in the plants treated with the new composition. The obtained information demonstrates the possibility of mitigating the adverse effects of drought on the physiological response and production by applying antioxidants.

Keywords: growth; photosynthesis; physiologically active substances; plants; productivity; resistance; transpiration

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