2024

Cover story

Ensuring food safety requires effective contaminant monitoring in fruits. This study compares two methods used to measure the concentrations of Cu, Zn, Ni, Pb, and Cd in plums from conventional and ecological orchards. Results indicate that microwave-assisted digestion provides greater accuracy and efficiency than conventional methods, reducing contamination risks and improving metal recovery.

Studies conducted at “Ion Ionescu de la Brad” University of Life Sciences in Iași confirm that the analyzed plums meet international food safety standards set by FAO/WHO. Detected levels were low, with essential metals such as Cu and Zn found in optimal concentrations.

These findings highlight the importance of advanced analytical methods in assessing food quality and safety and contribute to the development of more precise standards for monitoring heavy metals in agricultural production.

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Growth and seed yield responses of two soybean (Glycine max L. Merr.) varieties to coconut water priming

Paul Kweku Tandoh, Irene Akua Idun, Bridget Yayra Bemanu, Abiboy Kofi Nyagblordzro, Solomon Kwaah, Akosua Damtuah

ABSTRACT. Soybean is an essential legume that is increasingly valued for its nutritious oil and protein contents. Despite the benefits derived from soybean, low seed viability and inconsistent germination are major production setbacks. The objective of this study was to determine the effect of seed priming agents on the growth and yield of two soybean varieties (Favour and Afayak). The research used a 2 × 5 factorial randomised complete block design with three replications. The soybean variety at 2 levels was the first factor (Favour and Afayak), and the second factor involved 5 priming treatments: 4 h of water (20°C), 6 h of water, 4 h of coconut water, 6 h of coconut water, and no priming. Coconut water priming significantly enhanced germination, with the Favour variety achieving the highest rate (75.5%) after 4 h of priming. Water priming for 4 h was most effective in enhancing plant height, stem girth and leaf development in the Afayak variety. Yield was maximised in Afayak primed with coconut water for 6 h (2520 kg/ha), while Favour primed for 4 h produced the highest number of pods (135.5). Regression analysis revealed that the growth parameters and germination percentage accounted for 95% of seed yield variation. The study concludes that coconut water is an effective priming agent, enhancing germination, growth and yield and recommends 4-h coconut water priming for Favour and 6-h priming for Afayak to optimise production.

Keywords: coconut water; germination; seed yield; soybean seed; vegetative growth.

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Comparative analysis of digestion methods for quantifying heavy metals in plum orchards

Mariana Rusu, Irina-Gabriela Cara, Manuela Filip, Denis Țopa, Gerard Jităreanu

ABSTRACT. Increasing interest in healthy food among the population raises concerns about heavy metals in fruit and their impact on public health. To assess this issue, this study presents a comparative analysis of digestion methods for quantifying heavy metals in plum orchards managed under conventional and ecological practices in the “Adamachi” Farm district of Iasi University of Life Sciences (IULS). We evaluated and optimised two wet digestion methods – in an open system and microwave-assisted – to determine the concentrations of heavy metals, such as copper (Cu), zinc (Zn), nickel (Ni), lead (Pb), and cadmium (Cd), which pose environmental and health risks. The metal concentrations were measured using an atomic absorption spectrophotometer, according to standard methods. Microwave-assisted digestion was more efficient and faster than the conventional method (in an open system). The ecologically managed orchards showed a lower heavy metal content overall, with the exception of Cu levels, due to the Cu-based treatments. By determining the estimated daily intake (EDI), target hazard quotient (THQ), and hazard index (HI) for both children and adults, the potential health risks from heavy metals were determined. There were no related associated risks to human health (THQ and HI < 1), and the accumulated metals in plum fruit samples showed that the EDI values followed the descending order of Cu > Zn > Ni > Pb > Cd. The analysis revealed non-significant differences for most data obtained after processing using the two methods. These results highlight the importance of selecting the optimal digestion methods for heavy metal analysis in plums and sustainable agricultural practices to safeguard the environment and consumer health from heavy metal contamination.

Keywords: health risk; heavy metals; plum orchard; wet digestion.

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Plant diversity at selected dumpsites in Abakaliki: exploring species tolerance and carbon storage functions

Onyinyechi Priscilla Okpara, Okechukwu Idumah Okogwu

ABSTRACT. The aim of this study was to determine the species diversity and carbon storage potential of herbaceous plants growing within selected dumpsites in the Abakaliki metropolis. The line transect method was used to collect plants from five study stations: Waterworks (A), Kpirikpiri (B), Newlayout (C), FETHA (D) and Presco (E). At each study station, a 15 m×30 m area was measured using tape and demarcated with pegs and ropes. The identified species were collected, the biomass, species frequency, density, dominance, diversity and carbon uptake were measured. The diversity indices evaluated showed that active dump station A was the most diverse and evenly distributed site (Shannon–Weiner diversity index: A=2.43, B=1.09, C=1.16, D=1.14, E=0.99, Pileou’s evenness index, A=1.53, B=0.70, C=0.68, D=0.66, E=0.69). Additionally, at station D, Simpson’s dominance (A=0.27, B=0.32, C=0.24, D=0.43, E=0.10) and Magalef (species richness) indices (A=1.7, B=4.07, C=3.75, D=4.20, E=3.38) were the highest. The herbaceous Ghomphrena celeisoides had the highest relative dominance, relative frequency and important value index across the studied stations. Analysis of variance of the carbon uptake potentials of species showed significant values for abandoned dump stations C (Newlayout) and D (FETHA) when p<0.05. Therefore, total sequestered carbon in stations C (F=15.97, p<0.001) and D (F=8.33, p<0.001) and sequestered carbon dioxide equivalent at stations C (F=15.96, p<0.001) and D (F=43.68, p<0.001) were significant at 95% confidence level. The results indicate that species at dormant waste disposal sites sequester significant amounts of carbon; therefore, they are recommended for the phytoremediation of disturbed or destroyed ecosystems. Keywords: carbon uptake; density; diversity; relative frequency.

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Health risk assessment of lead in dairy products from various environments in Egypt

Mahmoud Abou Donia, Assem Abou-Arab, Ali Enb

ABSTRACT. Milk and dairy products are nutritious foods that are beneficial to human health at all life stages and provide essential nutrients required by the body. However, despite their importance, these products are susceptible to contamination by toxic environmental pollutants, such as lead (Pb), originating from surrounding environmental sources. This study aimed to evaluate the potential health risks associated with Pb exposure in adults and children through the consumption of contaminated milk and dairy products from various locations in Egypt using estimated daily intake, target hazard quotient, and excess cancer risk metrics. The data indicated that the daily intake (DI) of Pb in the raw milk samples ranged from 131.6 to 557.9 µg in industrial areas, 107.7 to 456.6 µg in traffic areas, and 35.8 to 151.7 µg in rural areas. In contrast, the DI of Pb in sterilised milk ranged from 19.3 to 82.0 µg in industrial areas, 17.9 to 75.7 µg in traffic areas, and 16.0 to 67.8 µg in rural areas. For processed cheese, values ranging from 2.0 to 2.4, 2.5 to 2.9, and 2.4 to 2.8 µg were found across these areas, respectively. The mean DI values of Pb were 70.1, 59.7, and 11.5 µg for Domiati cheese samples and 112.2, 103.7, and 481.6 µg for Ras cheese from industrial, traffic, and rural areas, respectively. The measured health risk indices showed no significant risks associated with the consumption of the dairy products examined, except for raw milk, particularly when consumed in large quantities from industrial and traffic areas. Purchasing raw milk from contaminated industrial or traffic zones should be avoided to limit its consumption, particularly for children.

Keywords: dairy products; lead; risk assessment.

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Winter wheat yield depending on different soil tillage systems in short-term crop rotations under Black Sea region conditions

Svitlana Pochkolina, Alexander Melnyk, Inna Kohut, Viacheslav Rudenko, Serhiy Vlasenko

ABSTRACT. A crop rotation system with optimal placement and saturation of leading agricultural crops can improve the environmental conditions of the surrounding environment and increase the agricultural efficiency. Therefore, solving this task is relevant both scientifically and practically, especially in the current conditions of deteriorating environmental conditions in Ukraine. The development of environmentally safe technologies for the competitive production of high-quality crop products in the Black Sea Steppe. The primary method was fieldwork, supplemented by analytical studies, measurements, calculations, and observations according to generally accepted methodologies and guidelines in agriculture and crop production. This study focused on crop rotation systems and primary soil tillage systems. This study examined the impact of different primary soil tillage systems on the yield of winter wheat and oats in a short-rotation system. For the 1st and 4th crops, the most favourable conditions for winter wheat yield formation were observed when it was planted after black fallow and green manure fallow with winter vetch. In these cases, almost identical grain yields were recorded, averaging 3.98 and 4.08 t/ha for the 1st crop and 3.29 and 3.16 t/ha for the 4th crop. The differences in yield were not significant. For the 2nd crop, when comparing yield with the control (black fallow), an increase in yield was observed in the background of green manure fallow with winter vetch. The increase of 6.9% was statistically significant. The no-till system of primary soil cultivation provided the best conditions for the formation of winter wheat grain yield in the 1st, 2nd, and 4th crops, with increases of 10.4, 6.9, and 5.4%, respectively, compared to conventional tillage. In the experimental variants, for the 1st and 4th crops, green manure fallow with winter vetch affected winter wheat yield, almost at the level of black fallow. For the 2nd crop, green manure fallow with winter vetch showed a clear advantage. Across all winter wheat crops, a positive impact on yield formation was observed with the no-till system. This system resulted in the highest yield compared to other soil tillage systems.

Keywords: black fallow; green manure fallow; oats; soil tillage systems.

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Investigating the potential use of plant-based compounds as environmentally friendly management strategies for controlling root knot nematodes in cabbage crops

Halimat Shola Baba, Oluropo Ayotunde Apalowo

ABSTRACT. Vegetables are infected by root knot nematodes, especially Meloidogyne incognita, which results in both quantitative and qualitative losses. This study’s objective was to assess the effectiveness of plant extracts from Senna alata and Tamarindus indica in controlling cabbage-infecting root knot nematodes. The experiment was carried out to determine the effect of aqueous and powdered extracts of T. indica and S. alata on the control of root knot nematodes in two cabbage varieties. The test plants underwent phytochemical screening. Data were gathered on plant height, shoot weight, root weight, yield and soil nematode populations. All numerical data were subjected to analysis of variance using the GENSTAT statistical programme. Treating F1 Majesty and F1 Minotaur with powdered T. indica extract resulted in a significantly better (p>0.05) growth and yield (62.77 cm and 53.77 cm) than the other treatments. Significant variations were found between the shoot, root and yield weights of treated plants and their control counterparts. The positive control had the highest number of galled roots in both varieties (6.25 and 8.27). The treated plants performed much better than the control plants. The experiment also revealed some compounds, such as hexadecanoic, butyric and octadecadienoic acid. Based on this study’s findings, it is advisable to make use of these botanicals (T. indica and S. alata) on root knot-infested farms, as they are cheap, available and eco-friendly alternatives to chemical nematicides, which are expensive and not environmentally friendly.

Keywords: infecting; phytochemical; screening; significant; varieties.

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Listeria monocytogenes in dairy products occurrence, monitoring and surveillance

Cristina Ștefania Afloarei, Amelia Buculei, Ancuța Chetrariu, Adriana Dabija

Cross-contamination with foodborne microorganisms is a challenge at every stage of food preparation. Listeria monocytogenes poses serious and persistent problems for the food industry because of its ability to withstand a broad range of temperatures and pH levels and thrive under high salt concentrations. These factors significantly increase the risks to consumers. Although Listeria is uncommon in the general population, the bacterium is frequently isolated from food and environmental sources. The prevalence of systemic listeriosis is notably higher among vulnerable groups, such as the elderly, pregnant women, and individuals with weakened immune systems. Among the species within the Listeria genus, L. monocytogenes is the most significant in food contexts because of its capacity for proliferation and its adaptability to changing environments. Advancements in detection technologies have enabled the identification of more outbreaks with fewer cases per incident. To monitor and validate the efficacy of control measures, robust environmental monitoring programmes are essential. These programmes include establishing protocols for sampling and detection, determining sampling frequency, selecting sampling zones, and implementing corrective actions. This study aims to review the specialist literature on the management, surveillance, and prevalence of L. monocytogenes in dairy products.

Keywords: food safety; health priority; microbial contamination; risk assessment.

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Serum protein and glucose of cocks fed CrPic diets with or without vitamin C at high ambient temperature

Francis Bosede Adebayo

ABSTRACT. This study assessed the effect of chromium picolinate (CrPic) and vitamin C (Vit C) on the serum proteins and glucose of two breeds of cocks. A total of 192 sexually mature cocks, 24 weeks old, consisting of 96 Noiler cocks (NC) and 96 White Leghorn cocks (WLC), were utilised in the study. The two breeds were allocated to 8 treatments and reproduced four times, with six cocks per replication, in a 2 × 2 x 4 factorial design. A total of 8 experimental diets were constituted with four diets containing CrPic at 0.00, 0.40, 0.80 and 1.20 mg CrPic/kg without Vit C and another four diets containing CrPic at 0.00, 0.40, 0.80 and 1.20 mg CrPic/kg with Vit C inclusion at 200 mg/kg diet in each of the treatments for 16 weeks. The data collected were subjected to a 2 × 2 x 4 factorial arrangement using SAS (version 9.2). The serum proteins studied included total protein (TP) (g/dl), albumin (ALB) (g/dl), and globulin (GLB) (g/dl). Supplementation of CrPic significantly (p<0.05) enhanced serum proteins up to 0.8 mg/kg diet. However, a 1.2 mg/kg diet significantly reduced serum protein concentrations. Serum glucose was not significantly (p>0.05) different among cocks fed varied CrPic supplements compared to the control diet. The immunostimulant activity of vitamin C at a 200 mg/kg diet increased serum protein and decreased glucose levels compared to the control. Notably, interactions between 0.8 mg/kg CrPic and 200 mg/kg vitamin C revealed breed-specific effects: It increased serum protein in both breeds, decreased serum glucose in White Leghorns, but increased it in Noilers. This suggests a differential effect of the CrPic and vitamin C combination on glucose regulation in these breeds. This study therefore suggests that a 0.8 mg CrPic/kg diet with Vit C has the potential to significantly increase serum protein and enhance insulin sensitivity in cocks raised at high ambient temperatures.

Keywords: chromium picolinate; glucose; protein; serum; vitamin C.

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Herbicide use in Nigeria: a review of its effects on human, animal and environmental health

Goodness O. Oyetunji, Emmanuel A. Olagunju, Oluwakemi O. Ajayi, Gabriel O. Adesina

ABSTRACT. Herbicides are a class of pesticide compounds with a specific role in weed control. Most herbicides have a positive effect on crop production; however, they are also harmful to the environment, animals, and humans when misused. The aims of this study were to identify commonly used herbicides in Nigeria, examine the effects of herbicides from the perspective of One Health (i.e., the health of humans, animals, and the environment), and increase public awareness of the negative impact of herbicide misuse on human, animal, and environmental health in Nigeria. We conducted a systematic literature search for this study using Google Scholar, the Bielefeld Academic Search Engine (BASE), Research Gate, and PubMed, focusing on research studies conducted in Nigeria. In total, 192 articles were included in this review. Atrazine, glyphosate, metolachlor, paraquat, and 2,4-D are the most commonly used herbicides in Nigeria. According to reports, some of these chemicals inhibit plant photosynthesis and disrupt the female luteinising hormone surge, which disrupts ovulation. Moreover, these chemicals can lead to negative outcomes, such as headaches, oxidative stress, and pollution. Only 1.0, 9.4, and 16.1% of the studies examined the impact of herbicides on human, animal, and environmental health, respectively. Similarly, only 11 studies (5.7%) investigated bioherbicide development in Nigeria, and only 2.6% tested for herbicide residues in crops. Nigeria desperately needs public education regarding the use of herbicides. One health intervention is urgently needed.

Keywords: herbicides; one health; public health; weeds.

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The Effect of Diversified Crop Rotations on Soil Organic Carbon Dynamics in a Clayey Tropical Soil

Chinelo Jennifer Nwaiwu, Beata Emoke Madari, Marcia Thais De Melo Carvalho, Priscila Silva Matos, Akudo Ogechukwu Onunwa, Chike O. Madueke, Emmanuel Chinweike Nnabuihe, Maduabuchi Johnbosco Okafor, Tochukwu Victor Nwosu

ABSTRACT. This study assessed the impact of crop diversification within no-till crop rotations on soil organic carbon and total nitrogen stocks, as well as on labile (Carbon and Nitrogen in the particulate organic matter) and persistent (Carbon and Nitrogen in the mineral-associated organic matter) Organic matter fractions. The objective was to identify practical indicators for monitoring public policies promoting low carbon emission agriculture. Field experiment was conducted in 2014/2015 cropping season using a complete random block design. Seven treatments were evaluated: soybean/cotton (CONTROL); maize/soybean (M/Sb); soybean/maize+brachiaria (Sb/M+Br); soybean/millet+brachiaria/crotalaria spectabiliscotton (Sb/Mt+Br/CrsCt); soybean/cotton/common beans/millet+ brachiaria (Sb/Ct/Cb/Mt+Br); millet-cotton/ soybean/maize/crotalaria spectabilis (Mt-Ct/Sb/M/Crs); crotalaria-cotton/soybean/ sorghum+brachiaria/crotalaria ochroleuca+ brachiaria (Cr-Ct/Sb/Sg+Br/Cro+Br). Sampling was done in May 2020 on an Oxisol in a neotropical savanna of the Central West region of Brazil (Capivara Experimental Research Farm of Embrapa Rice and Beans, Santo Antônio de Goiás, Goiás State, Brazil). Treatment comparisons were made after correction for equivalent soil mass per soil layer. The C-POM, N-POM, C-MAOM, and N-MAOM fractions were obtained through granulometric physical fractionation. Total SOC and SOC stocks were inadequate indicators of the impact of crop rotations on SOC. However, the distribution of C and N among the soil organic matter (SOM) fractions (C-POM, N-POM, C-MAOM, and N-MAOM) was influenced by crop rotations. Rotations with greater crop diversity, including gramineae, had higher concentration of C and N in the particulate SOC (C-POM and N-POM). Differences in rotation composition also affected the C to N ratio, particularly in the POM fraction, which was higher in rotations involving brachiaria grass and maize. Most diversified rotations contributed to maintaining higher C-POM stocks.

Keywords: carbon and nitrogen stocks; crop rotation; mineral-associated organic matter (MAOM); Oxisol; particulate organic matter (POM); zero-tillage.

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Cover Story

Erythrina lysistemon, commonly known as the South African coral tree, is an ornamental species valued for its ecological, agricultural, and medicinal benefits. However, its seeds often exhibit physical dormancy due to an impermeable seed coat, preventing germination even under favorable conditions. This study evaluates the effectiveness of two primary methods – mechanical scarification and soaking treatments- on freshly collected seeds and those stored for 12 months.

The findings reveal that mechanical scarification significantly improves germination rates, reducing the time required for seedlings to emerge. While soaking treatments also enhanced germination in some cases, they often resulted in high seed mortality. Notably, seeds stored for 12 months exhibited lower germination rates and delayed sprouting under all treatments, with soaking proving ineffective in overcoming dormancy.

These insights highlight the importance of selecting appropriate techniques for seed propagation, particularly for reforestation and ecological restoration initiatives. Mechanical scarification stands out as the most effective method for breaking dormancy and ensuring successful establishment of E. lysistemon seedlings.

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