Journal of Applied Life Sciences and Environment (ALSE) is a peer-reviewed, open-access journal on Agricultural, Horticultural and Animal Sciences, Food Sciences, Veterinary Medicine and Environmental Management in the field of Applied Life Sciences, published quarterly online and in print by “Ion Ionescu de la Brad” Iasi University of Life Sciences (IULS).

Submit to ALSEReview for ALSE

Latest Articles

Efficacy of Aqueous and Powdered Leaf Extracts Of Chromolaena odorata (Asterales: Asteraceae) and Ficus mucuso (Rosales: Moraceae) Botanicals on Root-Knot Nematode Infecting Watermelon in Kwara State, Nigeria

Oluropo Ayotunde Apalowo, Nkechi Betsy Izuogu, Halimat Shola Baba, Isaiah Olusesan Adepoju, C.M. Olajide, Muhammed Adewole Adeyemi, Olusegun Samuel Balogun

ABSTRACT. Watermelon production in Kwara State, Nigeria, is affected by root-knot nematode (RKN), as all varieties of the crop are susceptible. The aim of this study was to identify readily available and environmentally safe nematicides for the management of RKN. The field was located at the Teaching and Research Farm of the University of Ilorin and set out in a randomised complete block design, with four replications. Aqueous and powdered extracts of Chromolaena odorata (L) King and Robinson and Ficus mucuso Welw. ex Ficalho were applied alone and in combination. There were seven total treatments: C. odorata aqueous, F. mucuso aqueous, C. odorata powder, F. mucuso powder, C. odorata and F. mucuso aqueous, C. odorata and F. mucuso powder, and the control. Data were collected on growth, yield and nematode populations. All data collected were subjected to analysis of variance, and treatments were compared using Duncan’s multiple range test at a 5% level of significance. The essential oil of each botanical was determined using gas chromatography-mass spectrometry. The vine length (185.61) and yield (2401.05) of plants treated with botanicals were significantly higher than those of the control at P<0.005. The nematode population was also significantly lower in plants treated with botanicals than in the control (318.30 and 230.00, first and second year, respectively) at P<0.005. Among the treatments, the combination of C. odorata and F. mucuso powders was the most effective, with higher growth and yield performance. The experiment showed that aqueous and powdered extracts of C. odorata and F. mucuso were effective in managing RKN in the field. Keywords: Botanicals; C. odorata; extracts; F. mucuso; watermelon.

read more

Effect of Different Leonardite Doses and Cross-Sowing Methods on the Growth and Yield of Red Lentil

Betül Kolay, Ali Rıza Öztürkmen

ABSTRACT. This study was carried out to determine the effect of different cross-sowing methods and different doses of leonardite on the growth of red lentils under rain-fed conditions. Treatments consisted of different cross-sowing methods (control, 45° cross-sowing and 90° cross-sowing) and different leonardite doses (0, 500, 1000, 1500, 2000 and 2500 kg ha−1). The trial was carried out in Diyarbakır province in Türkiye. The same rate of leonardite was applied to the same plots for two consecutive years to observe the two-year effect of leonardite in the experiment, which was established with four replications according to the factorial experimental design in the 2018-2019 and 2019-2020 production seasons. A positive effect of leonardite application was observed on the number of nodules and fresh root weight in the first production season. The highest nodule number (11.84) was obtained with 2500 kg ha−1 and the highest fresh root weight (0.24, 0.25 and 0.24 g) was obtained at 500, 1000 and 1500 kg ha−1 doses, respectively. The highest plant height, number of main branches, and number of pod parameters were obtained at a dose of 1500 kg ha−1 in the second production season (36.63 cm, 3.95 and 17.43, respectively). The highest grain yield (889.5 kg ha−1), dry root weight (0.125 g) and number of nodules (50.01) were obtained at a dose of 1000 kg ha−1 in the second production season. It was determined that different sowing methods did not affect grain yield in either production season.

Keywords: cross-sowing; leonardite; red lentils.

read more

The Contributions of Wildlife to People’s Livelihoods and Economic Development in Sub-Saharan Africa

Jesse Zvikonyaukwa, Kudakwashe Musengi, Clarice P. Mudzengi, Andrew Tapiwa Kugedera

ABSTRACT. Wildlife has the potential to support people’s livelihoods and economic development in many African countries. The objective of the review was to evaluate the potential contribution of wildlife to people’s livelihoods and economic development in Africa. Several databases were searched to identify articles that have explored the contributions of wildlife to people’s livelihoods and economic development. The results indicate that wildlife contributes both consumptive and non-consumptive resources towards people’s livelihoods, with bush meat being the greatest consumptive contribution and employment the greatest non-consumptive contribution. Revenue collected from tourists, trophy hunting, and game viewing have been used for infrastructure and rural development. However, wildlife has declined in many African countries due to land redistribution, drought, habitat fragmentation, human population growth, and illegal hunting. Setting up law enforcement agents and creating community-based wildlife management could restore the benefits of wildlife.

Keywords: bush meat; edible fruits; game viewing; trophy hunting; wildlife resources.

read more

Cover story

The intensity of selection, inadvertent pathways of domestication, and the influence of climate change collectively amplify the frequency of detrimental alleles. This phenomenon, in turn, triggers genetic drift, leading to an inadvertent decline in the diversity of plant genetic resources. Genetic variability is of utmost importance for a species’ adaptability and overall fitness. Our investigation centres on Triticum germplasm within the agroclimatic conditions of the Suceava Gene Bank and the Ezareni farm of Iasi University of Life Sciences, Romania, encompassing 2021 – 2022 trials. Comprehensive morphophysiological characterisation was conducted across 200 cultivated varieties, spanning three Triticum species (T. aestivumT. turgidum and T. monococcum) (see page 289).

read more

Comparative Analysis of Ecosystem Service Delivery Between Two Coastal Lagoons in Ghana

Kofi Adu-Boahen

ABSTRACT. The integration of ES into global policies represents a significant step towards harmonising sustainability and growth and unifying dialogues on biodiversity and sustainable development to benefit humanity. Consistent assessment of the availability of ES is vital to enable ongoing monitoring and support sustainable natural resource management and decision-making. This study was conducted to comparatively analyse the ecosystem service delivery between two coastal lagoons in Ghana. The Millennium Ecosystem Assessment (MEA) and Marine Ecosystem Services Assessment Tool (MESAT) scales were used in the study. The paper was based on the positivist paradigm and employed inferential statistics with SPSS v23 for data analysis. The results indicate no statistical differences between the Fosu and Muni lagoons in terms of their ecosystem service delivery; the null hypothesis is accepted for the two ecosystem service scales used and confirms that Fosu and the Muni Lagoons are similar in their deliveries of ecosystem services. The relationship between ES and human well-being is complex due to the interplay between social and ecological systems. To address coastal issues and develop management plans, the study proposes using MESAT and MEA for evaluation and solution provisioning. It further recommends expanding stakeholder and decision-maker involvement in lagoon management to promote sustainability and enhance stakeholder participation in decision-making.

Keywords: cultural services; Muni and Fosu Lagoons; provisioning services; regulatory services; social-ecological system; supporting services.

read more

Seroprevalence of Small Ruminant Lentivirus Infection in a Goat Flock in Eastern Romania

Adriana Elena Aniță, Gheorghe Savuța, Dragoș Constantin Aniță

ABSTRACT. Small ruminant lentiviruses (SRLVs) pose significant economic challenges to goat farming and have a detrimental impact on animal welfare. The objectives of this study were to deepen our comprehension of SRLV infections and their implications for small ruminant farming. Within the studied herd, the detected seroprevalence of SRLV was notably high at 91.92% (95.0% CI: 86.5%–97.3%). This finding strongly suggests the enduring presence of SRLV infection within the goat farm, spanning multiple years. Antibodies against SRLVs were detected across all selected age categories and breeds, irrespective of the presence or absence of clinical signs. The data gleaned from this study allow significant implications, raising concerns about the potential for future clinical outbreaks resulting from SRLV infections in eastern Romanian goat herds. Given these findings, the imperative for the continuous surveillance of SRLV infections becomes evident. Ongoing vigilance and monitoring are essential to mitigate the risk of disease transmission, minimise economic losses and safeguard the welfare of goats within the region.

Keywords: caprine arthritis encephalitis; goat; lentivirus; Maëdi-Visna.

read more

Establishing the Distribution of West Nile Virus Outbreaks in Romania by Using Climatic Scenarios

Liviu Dan Miron, Larisa Maria Ivănescu, Raluca Mîndru, Simona Mătiuț, Gabriela-Victoria Martinescu, Ilie Bodale

ABSTRACT. West Nile virus (WNV) is a re-emerging zoonotic pathogen that represents a threat to both human and animal health. It is difficult to estimate the impact of WNV in the future, although many of the climatic factors influencing its spread have been identified. In this study, we used bioclimatic indices to estimate those periods that favour the growth of vector mosquito populations and the incubation periods for the virus. To this end, we studied the climatic changes in the Romanian regions where cases of WN infection have been reported. Simulations were carried out for 2100 based on long-term scenarios. Identifying the bioclimatic conditions which can cause WNV outbreaks in Romania is necessary to anticipate and thereby prevent future epidemics. However, no extraordinary weather events were registered in the years with WNV outbreaks which could explain such a high number of cases. Thus, in the High Scenario (which will occur if actions to control (GHG) gas emissions are not taken or implemented effectively), the hatching period is extended until November, with the risk that adult mosquitoes are active throughout the year, ensuring a high survival rate of the virus within mosquitoes. In addition, in the High Scenario, the transmission period of the virus is extended from April to October, which underlines the need to establish monitoring and control programmes for both mosquito populations and the spread of the virus among the animal and human populations.

Keywords: climatic change effects; mosquitoes; temperature long-term projection; West Nile outbreak predictions.

read more

Experimental Cultivation of Seaweed on the Coast of Cox s Bazar, Bangladesh: Identifying the Effects of Environmental Parameters on Seaweed Growth

Simul Bhuyan, Sayeed Mahmood Belal Haider, Mrityunjoy Kunda, Abid Husain, Enam Chowdhury, Venkatramanan Senapathi, K. Sivakumar, Manickam Elangovan

ABSTRACT. The current study was carried out at Rezu Khal to determine the ideal area for seaweed farming. Additionally, this investigation uncovered species of commercially productive and lucrative seaweed. Temperature, salinity, pH, dissolved oxygen (DO), conductivity, and Formazin Nephelometric Units (FNU) of surface water ranged from 20.9 to 26.2°C, 24 to 26.2‰, 6.45 to 8.5, 92 to 105%, 33,256 to 64,267 µS/cm, and 11.1 to 42.8, respectively. Phosphate-phosphorus concentrations in surface water were 2.6–7.6 mg/L, 0.04–0.12 mg/L for nitrate-nitrogen, 0.002–0.04 mg/L for nitrite-nitrogen, 0.15–0.83 mg/L for silica, and 0.13–0.28 mg/L for ammonia. Three seaweed species (Gracilaria lemaneiformis, Hypnea musciformes, and Sargassum oligocystum) were cultivated in the selected areas. Two methods (net and long-line) were used for the culture. In this study, 15–20 kg of G. lemaneiformis were harvested every 15 days using the net method. H. musciformes gained 4 to 12 kg every 15 days. Although S. oligocystum thrived nicely, it was challenging to maintain its viability. The findings of this study indicate that seaweed farming is feasible and coastal residents may participate in seasonal income-generating endeavours in coastal waters.

Keywords: culture; effects; physicochemical parameters; seaweed; Rezu Khal.

read more

Use of Coconut Husks to Facilitate the Removal of H2S From Biogas

Minza I. Selele, Shaaban M. Mgana

ABSTRACT. The study investigates the potential of coconut husks in purifying biogas, a common issue in the use of biogas due to the high costs and environmental impact of expensive purification technologies. The study used an experimental setup generating biogas from kitchen waste and analysed its composition using an industry-standard biogas analyser. Coconut husks could remove hydrogen sulphide (H2S), with a high percentage removal efficiency of 54% at a column length of 0.5 m from the inlet. Based on the results, 2 kg of coconut husk could purify 837 L of biogas, with a removal efficiency of approximately 62%, before exhaustion. This suggests that coconut husks can be used in biogas purification to remove H2S, which is the most challenging component as it can corrode metal parts when used at high concentrations above 750 ppm. Further research is needed on large-scale purification using coconut husks at high pressure to allow more gas adsorption by the purification media.

Keywords: adsorption capacity; biogas composition; biogas purification; coir pith; coir fibre.

read more

Accelerated Ageing Assessment of Bitumen as a Preservative for the Treatment of Gmelina arborea Wood

Jacob Mayowa Owoyemi, Olasunkanmi Joshua Ogunniyi

ABSTRACT. Concerns about the health and environmental risks linked with the use of preservatives, such as chromate copper arsenate (CCA), zinc chloride, mercuric chloride, and the oil-born preservative creosote, prompted the quest for the use of readily available bitumen as a wood preservative. Using samples that had been processed into dimensions of 20 × 20 × 60 mm, the durability and physical characteristics of Gmelina arborea wood treated with bitumen were evaluated. The samples were dried for 24 hours in an oven set to 103°C and treated with hot bitumen at a melting point of 270°C to ensure the flow and maximum penetration of the bitumen. The density showed mean values of 504.93 and 498.71 kg/m3 for the untreated and treated samples, respectively, with the treated samples recording lower values due to the thermal difference in the density distribution between the untreated and treated wood samples. The average weight loss due to leaching of the untreated and treated wood samples after soaking in cold water and hot water was 3.07% and 0.07%, 1.49% and 1.38% respectively for the treatment. The study confirmed the suitability of using bitumen as a preservative for treating G. arborea wood in an environment with extreme weather conditions without causing serious leaching, thereby exposing the wood to degrading agents.

Keywords: accelerated ageing; bitumen; Gmelina arborea; treated wood; wood preservative.

read more

Application of Multi-Criteria Decision Analysis to Support Land Use Decision-Making in Ankasa Conservation Area, Ghana

Lawrence Damnyag, Francis Tease, Alex Aboagye Bampoh, Jewel Andoh

ABSTRACT. Deforestation in Ghana is primarily caused by agriculture, and as such, there is a need to explore agricultural systems that promote forest and environmental conservation. Studies on decision support frameworks that can help farmers and landowners make informed optimal land use choices are limited in the country. This study attempts to fill the gap. We used survey questionnaire and focus group discussion to gather data from farmers and other stakeholders. Multi-Criteria Decision Analysis (MCDA) technique was employed to analyse the data. The results showed that agroforestry, with the highest ranking (probability of 0.98), is the optimal land use option. Coconut and oil palm land use options were perceived to be contributing to the degradation of the periphery of the Ankasa Conservation Area (ACA). Intensifying education and training will increase the uptake of agroforestry as an integrated multifunctional land use strategy in the ACA.

Keywords: agriculture; agroforestry; decision support system; Ghana; protected area.

read more

Cornhusk Powders as Adsorbents for Nitrites in Solution: A Thermodynamic and Kinetic Approach

Alina Elena Trofin, Elena Ungureanu, Iuliana Motrescu, Lucia Carmen Trincă, Denis Constantin Țopa, Diana Beatrice Eperjessy

ABSTRACT. The retention of nitrite ions in solutions of different concentrations by three cornhusks-based powders was analyzed. Natural cornhusk powder (NCHP), as waste obtained from local market, the alkalized cornhusk powder (ACHP) and the biochar from the original material (CHBC) have been characterized through scanning electron microscopy (SEM) and elemental composition EDAX – TEAM analysis (Energy dispersive analysis X-ray – Texture and ele-mental analytical microscopy) and tested for the removal of nitrite ions. The influence of initial nitrite concentration and contact time was studied under slow stirring rate conditions (150 rpm). For all three adsorbents both Freundlich and Langmuir isotherm equations described the process with R2 > 0.95, denoting physical adsorption and chemisorption on the surface. The estimated retained quantities (mg·g-1) determined from isotherms were 4.4783 (NCHP), 8.3542 (ACHP) and 8.7413 (CHBC). The Ho&McKay model was better adjusted to the adsorption data with R2 > 0.985, while the Lagergren model produced regression factors between 0.61 and 0.88. Considering the biggest concentration of nitrite solution of 50 mg·L-1 and the longest contact time of 150 minutes, the equilibrium capacity qe (mg·g-1) predicted by the Ho&McKay model for the considered adsorbents were: 4.5065 (NCHP), 8.5179 (ACHP) and 8.9445 (CHBC) compared to the obtained qt (mg·g-1) of 4.4384 (NCHP), 8.0685 (ACHP) and 8.5753 (CHBC). The nitrite uptake in the experiments reached a maximum of 2.2192 mg·g-1 on NCHP, 4.0342 mg·g-1 on ACHP and 4.2877 mg·g-1 on CHBC. Considering the cost-effective treatment steps, there is the possibility of valorising an important amount of waste as adsorbent materials.

Keywords: cornhusks powder; nitrite removal; waste valorisation.

read more

Impact of the COVID-19 Pandemic on Embryo Transfer Activities in Europe

Ștefan Gregore Ciornei, Dan Drugociu, Florin Nechifor, Petru Roșca

ABSTRACT. This review highlights to what extent the embryo transfer (ET) activity in Europe was affected by the COVID-19 pandemic. The ET represents a procedure of modern reproductive biotechnologies that is increasingly applied in farms animals. Commercial ET is applied to cattle and, albeit to a lesser extent, to other species. For this study, official data reported by each country to the European (AETE) and international (IETS) forums, regarding ET activities, were accessed. Each country has an official rapporteur, but the procedures are not mandatory. The reported data included the total number of ET sessions, the type of embryos obtained in vivo derived (IVD) or in vitro produced (IVP), the collection rate of oocytes and embryos and transferable embryos produced both in cattle and other species. For comparison, the data of two similar pre-pandemic (2017 and 2018) and pandemic (2019 and 2020) periods were analysed. The pandemic period (2019) started with a record decrease in all categories of ET, most likely because of less reports by veterinaries since reporting took place in May 2020, already pandemic time Europe, indicating a false negative decrease in Europe, the total ET activities reported decreased by 31.59%. In 2019, the in vivo ET procedures in cattle decreased by 26,421 sessions compared to 2018 (approximately 20%). Surprisingly, during the pandemic, in vivo ET in equines increased by almost 40%.

Keywords: covid; embryo transfer; IVD; IVP; pandemic.

read more

Phenotypic Variability Evaluation of Wheat Varieties from the Suceava Gene Bank Collection

Tiberiu Emilian Sârbu, Iulian Gabur, Dănuț Petru Simioniuc, Daniela Domnica Plăcintă, Danela Murariu, Violeta Simioniuc

ABSTRACT. The intensity of selection, inadvertent pathways of domestication, and the influence of climate change collectively amplify the frequency of detrimental alleles. This phenomenon, in turn, triggers genetic drift, leading to an in-advertent decline in the diversity of plant genetic resources. Genetic variability is of utmost importance for a species’ adaptability and overall fitness. Our investigation centres on Triticum germplasm within the agroclimatic conditions of the Suceava Gene Bank, encompassing 2021–2022 field trials. Comprehensive morphophysiological characterisation was conducted across 200 cultivated varieties, spanning three Triticum species (T. aestivum, T. turgidum and T. monococcum). Initially, the data underwent meticulous processing involving the computation of amplitude of variation, variance (s2), standard deviation (√s), and coefficients of variation (s%) for three pivotal agronomical traits: plant height, spikelets per spike, and total seeds per spike. Furthermore, an extensive cluster analysis was performed, encompassing multiple vital plant descriptors. The findings unveiled a remarkable dispersion of data, with standard deviation, amplitude of variation, and coefficient of variation collectively indicating substantial variability among the cultivated varieties. Within the same population, an intriguing observation emerged; of the 200 genotypes analysed, 83 exhibited immunity to Septoria tritici. Delving deeper into the statistical analysis, we identified two primary clusters within the population. Overall, a significant proportion of this germplasm showcased elevated phenotype scores, rendering them well-suited for further exploration as foundational material in pre-breeding initiatives.

Keywords: cluster analysis; morphophysiological traits; Septoria tritici; Triticum germplasm.

read more

Effect of Gliricidia sepium Leafy Biomass and NPK (15:15:15) Fertiliser on the Growth and Yield of Tomato, Solanum lycopersicum (L.)

Adams Latif Mohammed, Esther Korkor Nartey, Frank Addai, Samuel Arthur, Elvis Bawah

ABSTRACT. The use of organic amendments and appropriate nutrient management practices is essential for sustainable agricultural production. The present study investigated the effect of Gliricidia sepium leafy biomass and NPK (15:15:15) on the growth and yield of tomato plants (Solanum lycopersicum L.). A randomised complete block design with five treatments and four replicates was employed in this experiment. The treatments included T1 (Control), T2 (100% G. sepium leafy biomass), T3 (25% G. sepium leafy biomass + 75% NPK fertiliser), T4 (50% G. sepium leafy biomass + 50% NPK), T5 (100% NPK), and T6 (75% G. sepium leafy biomass + 25% NPK). The application of G. sepium leafy biomass and NPK had significant effects on tomato growth and yield (p˂0.05). The combined application of G. sepium leafy biomass and NPK (T4) and the sole application of G. sepium leafy biomass (T2) resulted in the highest plant height, stem girth, and fruit yield compared to the other treatments. Additionally, treatment with 75% Gliricidia leafy biomass and 25% NPK fertiliser (T6) resulted in a superior fruit weight compared to the other treatments. The application of G. sepium leafy biomass (T2) alone and NPK alone (T5) showed a significant improvement in plant growth parameters and fruit yield compared to the control (T1), although the effects were not as pronounced when combined with NPK (T4). The application of NPK fertiliser (T3) also positively influenced plant growth and yield, but the effects were lower compared to T2, T4, and T6. Further studies are warranted to optimise the application rates of G. sepium leafy biomass and NPK for different tomato cultivars and growing conditions.

Keywords: agroforestry tree species; crop nutrition; fertilisers; growth; tomato plant.

read more

Dynamics of Urban Landscape and Its Thermal Interactions with Selected Land Cover Types: A Case of Benin City, Nigeria

Oseyomon John Aigbokhan, Oludare Hakeem Adedeji, Abiodun Olusegun Oladoye, John Adebayo Oyedepo

ABSTRACT. In this study, the spatiotemporal dynamics of the urban environment and thermal environment of Benin City are analysed. The maximum likelihood algorithm for land use and land cover (LULC) analysis was used to categorise Landsat images. The relative transfer equation (RTE) and land surface emissivity (LSE) approaches were used to retrieve the land surface temperature (LST), whereas the Cellular Automata-Markov (CA-Markov) algorithm was used to forecast the LULC for 2030. The findings reveal evolving LULC patterns over time. Built-up areas made up 19.66% of the total area in 1990, bare ground made up 9.25%, and vegetation made up 71.08%. Built-up areas reached 23.40% in 2000, bare land reached 12%, and the vegetation cover dropped to 64.16%. In 2010, there was an increase in the proportion of built-up areas to 44.38%, the proportion of bare land increased to 22.20%, and the proportion of vegetation decreased to 33.42%. Built-up areas reached 61.79% in 2020, compared to 22.29% for bare land and 61.79% for vegetation. Regarding the relationship between the fractional vegetation cover (FVC) and LST, for the years 1992, 2002, 2012, and 2022, R2 is equal to 0.87097, 0.84598, 0.83957, and 0.71838, respectively. Conversely, for the LST and the normalised difference built-up index (NDBI), the R2 values were 0.5975, 0.73876, 0.86615, and 0.90368 for 1992, 2002, 2012, and 2022 respectively. In conclusion, this study evaluates Benin City’s metropolitan setting and thermal environment. According to the LULC study, there are more built-up areas and less vegetation. The impact of the changing land cover on urban thermal features is shown through correlation analysis, which links more built-up regions to higher LSTs. These results can support urban design efforts to lessen the effects of climate change. Examining the distribution of the LST and its associations with particular land cover types was the major goal of this study. Future research will undoubtedly use this study as a useful reference when modelling urban terrain and temperature variations.

Keywords: algorithm; concentric zone; emissivity; zonal statistics.

read more

Seaweed Culture, Post-Harvest Processing, and Market Generation for Employment of Coastal Poor Communities in Cox s Bazar

Asrafi Md. Farhaduzzaman, Md. Suzan Khan, Mohammad Hasan, Rashedul Islam, Mahadi Hasan Osman, Md. Neamul Hasan Shovon, Sayeed Mahmood Belal Haider, Mrityunjoy Kunda, Md. Tarikul Islam, Md. Simul Bhuyan

ABSTRACT. Seaweed farming is one of the aquaculture industries with the greatest growth rate worldwide, with an annual production of over 33 billion tonnes, and a value of USD$11.8 billion which is predicted to treble by 2024. The current study was conducted in the Bakkhali River estuary and set out to survey the seaweed cultivation, processing, marketing status of the seaweed farmers and evaluate its financial benefits. Gracilaria lemaneiformis and Ulva intestinalis were cultured using the rope methods. The yield of G. lemaneiformis and U. intestinalis was good (Ten 15 m long ropes were used in each of the 45 plots, produced 7770 kg of G. lemaneiformis in the first 15 days while using ten 15 m long ropes were in each of the plots of U. intestinalis produced 2300 kg). Water parameters viz. temperature, salinity, pH, DO and transparency were checked every 15 days. According to previous literature, winter was determined to be the most productive time for seaweed development in Bangladesh. Seaweed was processed by the sun drying method and sold to retail clients, neighborhood shops and markets, and representatives in Cox’s Bazar, Bandarban, Rangamati and Khagrachari district, where G. lemaneiformis attracting the higher price. The income of seaweed farmers has increased more than in the previous years to maintain their family. Most of the farmers used raw seaweed as traditional medicine for the treatment of gastric problem (80%), diabetes (5%), blood pressure (3%) and cancer (2%). They also used seaweed as an additive with other vegetables, mutton and chicken. Education for coastal people is very difficult, but after cultivating seaweed, about 60% of farmers can afford their children’s education cost and about 85% of the households had electric fans, while the majority had mobile phones. The cost-benefit analysis demonstrated that seaweed cultivation is highly profitable.

Keywords: coastal communities; employment generation; marketing; processing; seaweed culture.

read more

Prevalence of West Nile Virus Antibodies in Indoor Dogs From an Urban Area in Iași, Romania: Indicators of Viral Presence and Urban Transmission Potential

Luanda Elena Oșlobanu, Luciana Alexandra Crivei, Ioana Alexandra Rățoi, Ioana Cristina Crivei, Gheorghe Savuța

ABSTRACT. West Nile Virus (WNV), a zoonotic mosquito-borne virus (mobovirus) originally isolated from the blood of a febrile Ugandan woman in 1937, caused substantial human disease in Europe starting in the 1990s and emerged in 1999 in The United States of America (USA) for the first time. It has become an important concern for public health due to its reemergence and frequent human outbreaks. The enzootic transmission cycle of arboviruses involves primary wild animals; however, spillover transmission is reported frequently in domestic animals. Dogs are dead-end hosts in WNV transmission epidemiology. However, detecting WNV antibodies in the dog population can indicate the virus’s presence and spread in different areas. The virus is known to be endemic in parts of Romania, including Iași County. The study aimed at assessing the prevalence of anti-WNV antibodies in indoor dogs from an urban area in Iași, where all the conditions for virus transmission are met (wetland, density of wildlife hosts including birds, abundance of vectors, domestic mammal hosts and synanthropic birds). Using a commercial enzyme-linked immunosorbent assay (INGEZIM West Nile COMPAC, Ingenasa, Madrid, Spain), serum samples collected from indoor dogs between 2020–2022 were screened for WNV antibodies. The results showed an overall seroprevalence of 12.2%. Detection of specific antibodies in dogs suggests a possible establishment of an urban cycle for WNV or other antigenically related flaviviruses.

Keywords: dogs; seroprevalence; West Nile.

read more

The Influence of Climatic Conditions on the Occurrence of Large Forest Fires: A Case Study of Stara Planina Nature Park in 2007 and 2019

Stanimir Živanović, Milena Gocić

ABSTRACT. This work investigates the relationship between the occurrence of large forest fires (more than 1,000 ha) and meteorological data. Specifically, it analyzes the characteristics of large forest fires in July 2007 (2,500 ha) and October 2019 (2,108 ha) in Serbia’s Stara Planina Nature Park (NP). The results show that at these times, forest fires occurred during long heatwaves with low relative humidity and high air temperature. Dry climatic conditions in the preceding months also contributed to the occurrence of large forest fires, alongside the presence of wind. The study uses climate indices based on the combination of air temperature and precipitation. Forest drought index (FAI) data show that 2007 and 2019 were drier than the 1961–1990 climate period. According to the Lang Precipitation Factor Index (AILang), the periods June–July 2007 and July–October 2019 can be classified as dry. The De Martonne Drought Index (ImDM) shows that July 2007 and October 2019 had the characteristic of areism. These were also the periods when large wildfires were recorded. The results of this study could be used for wildfire risk assessment in protected areas and for fire prevention and suppression planning.

Keywords: climate indices; large forest fire; Serbia; Stara Planina Nature Park.

read more

The Influence of The Usual Holding Solutions on Hyacinthus orientalis Cut Flower Vase Life

Lucian Răus, Mariana Volf, Diana Elena Bolohan

ABSTRACT. In this study, four holding solutions very often used by florists and the final consumer were tested to analyse their impact on the development of the inflorescence, as well as the elongation, weight and degree of bending of the hyacinth flowers. The use of these solutions has a general character, and the flower species react differently due to both their genetics and the conditions in which they were cultivated, handled and stored. To verify the effectiveness of the preservative solutions on Hyacinthus orientalis vase life, four solutions were prepared with 2% sucrose and none or one of the follow-ing antimicrobial substances: sodium hypochlorite, acetic acid and ascorbic acid. During the study, measurements were made on the weight, elongation of the floral stems, chlorophyll content of the leaves and vase life days. The vase life of the hyacinths in this experiment was 5 days, except for the flowers from the 2% sucrose holding solution, which started to wilt on day 5. On day 6, the flowers showed signs of senescence. The increase in the length of the inflorescence stem showed significant differences for the sucrose + ascorbic acid holding solution, with a maximum of 0.8 cm on day 4. However, the interest was not to maximise the elongation of the floral stem but to slow down this process since elongation leads to the bending of the stem, causing it to require additional support.

Keywords: antimicrobial substances; holding solutions; inflorescence stem elongation.

read more

Bibliometric Analysis of the Incidence of Non-Covid Respiratory Disease under NPI Conditions During the Pandemic

Nji T. Ndeh, Yacob T. Tesfaldet, Oluseye Oludoye

ABSTRACT. Publications associated with the incidence of respiratory diseases in light of the COVID-19 pandemic have skyrocketed. Using bibliometric analysis, this study assessed the characteristics of published articles regarding non-pharmaceutical interventions (NPIs) on other respiratory infections during the COVID-19 pandemic. On July 06, 2023, a systematic review of literature on the incidence of respiratory diseases was conducted using the Scopus database. VOS viewer software was used to visualize the network. This study yielded a total of 910 articles, which were eventually narrowed down to 148 articles. The findings of the bibliometric analysis revealed a trend in research that included not only the investigation of the incidence of other respiratory diseases but also that of gastrointestinal infections during the COVID-19 pandemic. Most of the research output was created by American and Chinese authors. Kim J. H. was the most productive and cited researcher, with three research articles. There is a scarcity of data on the effectiveness of NPI in preventing the spread of other respiratory diseases among various age groups, including children, adults, and vulnerable populations. Countries should consider increasing their support for COVID-19 research, to generate knowledge pertaining to NPIs and, by extension, other infectious diseases with similar transmission mechanisms.

Keywords: bibliometrics; COVID-19; incidence; keyword analysis; nonpharmaceutical interventions; respiratory disease.

read more

Physiological and Biochemical Aspects of Optimising the Sowing Rate of Spring and Winter Pisum sativum Forms

Victor Shcherbakov, Vyacheslav Rudenko

ABSTRACT. The winter and spring varieties of peas (Pisum sativum) require careful study of the entire range of technological measures, including seeding rates, as the basis for optimal sowing density and the formation of phytocenose crops. This issue has not been resolved to date, not only in the context of agrobiological justification but also in everyday practice in Ukraine. This research was carried out in a field experiment in a three-way factorial experiment: factor “A” was the type of development – winter and spring; factor “B” was the variety – spring peas (Svit and Darunok Stepu) and winter peas (Moroz, Enduro and Baltrapp); and factor “C” was the seeding rate. According to the field studies, differences in the physiological and biochemical parameters of the P. sativum test culture characterised the photosynthetic activity of the plants, considering the type of development and variety. The winter varieties of P. sativum were characterised by a higher chlorophyll content (by 35–40%) compared to the spring varieties, which had an economic effect with an increase in the yield of dry biomass of the experimental crop and a decrease in seeding rates, with the formation and increase in grain yield by 14–18%. The intensity of chlorophyll in the process was not a determining factor in the accumulation of organic biomass. The extensive nature of the integration complex was noted (the amount of chlorophyll – the amount of biomass). At the optimal seeding rate, a certain specificity was observed in different types of P. sativum: for spring varieties, the sufficient rate was 0.9 million seeds/ha, and for winter varieties, it was 0.7 million seeds/ha. For varieties with a low productivity level (spring – Svit and winter – Moroz), the optimum sowing rate was around 0.7 million seeds/ha. The increase in the seeding rate was accompanied by a decrease in the content of various chlorophyll fractions from 10–12% to 20–26%.

Keywords: chlorophyll; economic effect; Pisum sativum; productivity; seeding rate; varieties and types of development.

read more

Latest Articles

Efficacy of Aqueous and Powdered Leaf Extracts Of Chromolaena odorata (Asterales: Asteraceae) and Ficus mucuso (Rosales: Moraceae) Botanicals on Root-Knot Nematode Infecting Watermelon in Kwara State, Nigeria

Oluropo Ayotunde Apalowo, Nkechi Betsy Izuogu, Halimat Shola Baba, Isaiah Olusesan Adepoju, C.M. Olajide, Muhammed Adewole Adeyemi, Olusegun Samuel Balogun

ABSTRACT. Watermelon production in Kwara State, Nigeria, is affected by root-knot nematode (RKN), as all varieties of the crop are susceptible. The aim of this study was to identify readily available and environmentally safe nematicides for the management of RKN. The field was located at the Teaching and Research Farm of the University of Ilorin and set out in a randomised complete block design, with four replications. Aqueous and powdered extracts of Chromolaena odorata (L) King and Robinson and Ficus mucuso Welw. ex Ficalho were applied alone and in combination. There were seven total treatments: C. odorata aqueous, F. mucuso aqueous, C. odorata powder, F. mucuso powder, C. odorata and F. mucuso aqueous, C. odorata and F. mucuso powder, and the control. Data were collected on growth, yield and nematode populations. All data collected were subjected to analysis of variance, and treatments were compared using Duncan’s multiple range test at a 5% level of significance. The essential oil of each botanical was determined using gas chromatography-mass spectrometry. The vine length (185.61) and yield (2401.05) of plants treated with botanicals were significantly higher than those of the control at P<0.005. The nematode population was also significantly lower in plants treated with botanicals than in the control (318.30 and 230.00, first and second year, respectively) at P<0.005. Among the treatments, the combination of C. odorata and F. mucuso powders was the most effective, with higher growth and yield performance. The experiment showed that aqueous and powdered extracts of C. odorata and F. mucuso were effective in managing RKN in the field. Keywords: Botanicals; C. odorata; extracts; F. mucuso; watermelon.

read more

Effect of Different Leonardite Doses and Cross-Sowing Methods on the Growth and Yield of Red Lentil

Betül Kolay, Ali Rıza Öztürkmen

ABSTRACT. This study was carried out to determine the effect of different cross-sowing methods and different doses of leonardite on the growth of red lentils under rain-fed conditions. Treatments consisted of different cross-sowing methods (control, 45° cross-sowing and 90° cross-sowing) and different leonardite doses (0, 500, 1000, 1500, 2000 and 2500 kg ha−1). The trial was carried out in Diyarbakır province in Türkiye. The same rate of leonardite was applied to the same plots for two consecutive years to observe the two-year effect of leonardite in the experiment, which was established with four replications according to the factorial experimental design in the 2018-2019 and 2019-2020 production seasons. A positive effect of leonardite application was observed on the number of nodules and fresh root weight in the first production season. The highest nodule number (11.84) was obtained with 2500 kg ha−1 and the highest fresh root weight (0.24, 0.25 and 0.24 g) was obtained at 500, 1000 and 1500 kg ha−1 doses, respectively. The highest plant height, number of main branches, and number of pod parameters were obtained at a dose of 1500 kg ha−1 in the second production season (36.63 cm, 3.95 and 17.43, respectively). The highest grain yield (889.5 kg ha−1), dry root weight (0.125 g) and number of nodules (50.01) were obtained at a dose of 1000 kg ha−1 in the second production season. It was determined that different sowing methods did not affect grain yield in either production season.

Keywords: cross-sowing; leonardite; red lentils.

read more

The Contributions of Wildlife to People’s Livelihoods and Economic Development in Sub-Saharan Africa

Jesse Zvikonyaukwa, Kudakwashe Musengi, Clarice P. Mudzengi, Andrew Tapiwa Kugedera

ABSTRACT. Wildlife has the potential to support people’s livelihoods and economic development in many African countries. The objective of the review was to evaluate the potential contribution of wildlife to people’s livelihoods and economic development in Africa. Several databases were searched to identify articles that have explored the contributions of wildlife to people’s livelihoods and economic development. The results indicate that wildlife contributes both consumptive and non-consumptive resources towards people’s livelihoods, with bush meat being the greatest consumptive contribution and employment the greatest non-consumptive contribution. Revenue collected from tourists, trophy hunting, and game viewing have been used for infrastructure and rural development. However, wildlife has declined in many African countries due to land redistribution, drought, habitat fragmentation, human population growth, and illegal hunting. Setting up law enforcement agents and creating community-based wildlife management could restore the benefits of wildlife.

Keywords: bush meat; edible fruits; game viewing; trophy hunting; wildlife resources.

read more

Cover story

The intensity of selection, inadvertent pathways of domestication, and the influence of climate change collectively amplify the frequency of detrimental alleles. This phenomenon, in turn, triggers genetic drift, leading to an inadvertent decline in the diversity of plant genetic resources. Genetic variability is of utmost importance for a species’ adaptability and overall fitness. Our investigation centres on Triticum germplasm within the agroclimatic conditions of the Suceava Gene Bank and the Ezareni farm of Iasi University of Life Sciences, Romania, encompassing 2021 – 2022 trials. Comprehensive morphophysiological characterisation was conducted across 200 cultivated varieties, spanning three Triticum species (T. aestivumT. turgidum and T. monococcum) (see page 289).

read more

Comparative Analysis of Ecosystem Service Delivery Between Two Coastal Lagoons in Ghana

Kofi Adu-Boahen

ABSTRACT. The integration of ES into global policies represents a significant step towards harmonising sustainability and growth and unifying dialogues on biodiversity and sustainable development to benefit humanity. Consistent assessment of the availability of ES is vital to enable ongoing monitoring and support sustainable natural resource management and decision-making. This study was conducted to comparatively analyse the ecosystem service delivery between two coastal lagoons in Ghana. The Millennium Ecosystem Assessment (MEA) and Marine Ecosystem Services Assessment Tool (MESAT) scales were used in the study. The paper was based on the positivist paradigm and employed inferential statistics with SPSS v23 for data analysis. The results indicate no statistical differences between the Fosu and Muni lagoons in terms of their ecosystem service delivery; the null hypothesis is accepted for the two ecosystem service scales used and confirms that Fosu and the Muni Lagoons are similar in their deliveries of ecosystem services. The relationship between ES and human well-being is complex due to the interplay between social and ecological systems. To address coastal issues and develop management plans, the study proposes using MESAT and MEA for evaluation and solution provisioning. It further recommends expanding stakeholder and decision-maker involvement in lagoon management to promote sustainability and enhance stakeholder participation in decision-making.

Keywords: cultural services; Muni and Fosu Lagoons; provisioning services; regulatory services; social-ecological system; supporting services.

read more

Seroprevalence of Small Ruminant Lentivirus Infection in a Goat Flock in Eastern Romania

Adriana Elena Aniță, Gheorghe Savuța, Dragoș Constantin Aniță

ABSTRACT. Small ruminant lentiviruses (SRLVs) pose significant economic challenges to goat farming and have a detrimental impact on animal welfare. The objectives of this study were to deepen our comprehension of SRLV infections and their implications for small ruminant farming. Within the studied herd, the detected seroprevalence of SRLV was notably high at 91.92% (95.0% CI: 86.5%–97.3%). This finding strongly suggests the enduring presence of SRLV infection within the goat farm, spanning multiple years. Antibodies against SRLVs were detected across all selected age categories and breeds, irrespective of the presence or absence of clinical signs. The data gleaned from this study allow significant implications, raising concerns about the potential for future clinical outbreaks resulting from SRLV infections in eastern Romanian goat herds. Given these findings, the imperative for the continuous surveillance of SRLV infections becomes evident. Ongoing vigilance and monitoring are essential to mitigate the risk of disease transmission, minimise economic losses and safeguard the welfare of goats within the region.

Keywords: caprine arthritis encephalitis; goat; lentivirus; Maëdi-Visna.

read more

Establishing the Distribution of West Nile Virus Outbreaks in Romania by Using Climatic Scenarios

Liviu Dan Miron, Larisa Maria Ivănescu, Raluca Mîndru, Simona Mătiuț, Gabriela-Victoria Martinescu, Ilie Bodale

ABSTRACT. West Nile virus (WNV) is a re-emerging zoonotic pathogen that represents a threat to both human and animal health. It is difficult to estimate the impact of WNV in the future, although many of the climatic factors influencing its spread have been identified. In this study, we used bioclimatic indices to estimate those periods that favour the growth of vector mosquito populations and the incubation periods for the virus. To this end, we studied the climatic changes in the Romanian regions where cases of WN infection have been reported. Simulations were carried out for 2100 based on long-term scenarios. Identifying the bioclimatic conditions which can cause WNV outbreaks in Romania is necessary to anticipate and thereby prevent future epidemics. However, no extraordinary weather events were registered in the years with WNV outbreaks which could explain such a high number of cases. Thus, in the High Scenario (which will occur if actions to control (GHG) gas emissions are not taken or implemented effectively), the hatching period is extended until November, with the risk that adult mosquitoes are active throughout the year, ensuring a high survival rate of the virus within mosquitoes. In addition, in the High Scenario, the transmission period of the virus is extended from April to October, which underlines the need to establish monitoring and control programmes for both mosquito populations and the spread of the virus among the animal and human populations.

Keywords: climatic change effects; mosquitoes; temperature long-term projection; West Nile outbreak predictions.

read more

Experimental Cultivation of Seaweed on the Coast of Cox s Bazar, Bangladesh: Identifying the Effects of Environmental Parameters on Seaweed Growth

Simul Bhuyan, Sayeed Mahmood Belal Haider, Mrityunjoy Kunda, Abid Husain, Enam Chowdhury, Venkatramanan Senapathi, K. Sivakumar, Manickam Elangovan

ABSTRACT. The current study was carried out at Rezu Khal to determine the ideal area for seaweed farming. Additionally, this investigation uncovered species of commercially productive and lucrative seaweed. Temperature, salinity, pH, dissolved oxygen (DO), conductivity, and Formazin Nephelometric Units (FNU) of surface water ranged from 20.9 to 26.2°C, 24 to 26.2‰, 6.45 to 8.5, 92 to 105%, 33,256 to 64,267 µS/cm, and 11.1 to 42.8, respectively. Phosphate-phosphorus concentrations in surface water were 2.6–7.6 mg/L, 0.04–0.12 mg/L for nitrate-nitrogen, 0.002–0.04 mg/L for nitrite-nitrogen, 0.15–0.83 mg/L for silica, and 0.13–0.28 mg/L for ammonia. Three seaweed species (Gracilaria lemaneiformis, Hypnea musciformes, and Sargassum oligocystum) were cultivated in the selected areas. Two methods (net and long-line) were used for the culture. In this study, 15–20 kg of G. lemaneiformis were harvested every 15 days using the net method. H. musciformes gained 4 to 12 kg every 15 days. Although S. oligocystum thrived nicely, it was challenging to maintain its viability. The findings of this study indicate that seaweed farming is feasible and coastal residents may participate in seasonal income-generating endeavours in coastal waters.

Keywords: culture; effects; physicochemical parameters; seaweed; Rezu Khal.

read more

Use of Coconut Husks to Facilitate the Removal of H2S From Biogas

Minza I. Selele, Shaaban M. Mgana

ABSTRACT. The study investigates the potential of coconut husks in purifying biogas, a common issue in the use of biogas due to the high costs and environmental impact of expensive purification technologies. The study used an experimental setup generating biogas from kitchen waste and analysed its composition using an industry-standard biogas analyser. Coconut husks could remove hydrogen sulphide (H2S), with a high percentage removal efficiency of 54% at a column length of 0.5 m from the inlet. Based on the results, 2 kg of coconut husk could purify 837 L of biogas, with a removal efficiency of approximately 62%, before exhaustion. This suggests that coconut husks can be used in biogas purification to remove H2S, which is the most challenging component as it can corrode metal parts when used at high concentrations above 750 ppm. Further research is needed on large-scale purification using coconut husks at high pressure to allow more gas adsorption by the purification media.

Keywords: adsorption capacity; biogas composition; biogas purification; coir pith; coir fibre.

read more

Accelerated Ageing Assessment of Bitumen as a Preservative for the Treatment of Gmelina arborea Wood

Jacob Mayowa Owoyemi, Olasunkanmi Joshua Ogunniyi

ABSTRACT. Concerns about the health and environmental risks linked with the use of preservatives, such as chromate copper arsenate (CCA), zinc chloride, mercuric chloride, and the oil-born preservative creosote, prompted the quest for the use of readily available bitumen as a wood preservative. Using samples that had been processed into dimensions of 20 × 20 × 60 mm, the durability and physical characteristics of Gmelina arborea wood treated with bitumen were evaluated. The samples were dried for 24 hours in an oven set to 103°C and treated with hot bitumen at a melting point of 270°C to ensure the flow and maximum penetration of the bitumen. The density showed mean values of 504.93 and 498.71 kg/m3 for the untreated and treated samples, respectively, with the treated samples recording lower values due to the thermal difference in the density distribution between the untreated and treated wood samples. The average weight loss due to leaching of the untreated and treated wood samples after soaking in cold water and hot water was 3.07% and 0.07%, 1.49% and 1.38% respectively for the treatment. The study confirmed the suitability of using bitumen as a preservative for treating G. arborea wood in an environment with extreme weather conditions without causing serious leaching, thereby exposing the wood to degrading agents.

Keywords: accelerated ageing; bitumen; Gmelina arborea; treated wood; wood preservative.

read more

Application of Multi-Criteria Decision Analysis to Support Land Use Decision-Making in Ankasa Conservation Area, Ghana

Lawrence Damnyag, Francis Tease, Alex Aboagye Bampoh, Jewel Andoh

ABSTRACT. Deforestation in Ghana is primarily caused by agriculture, and as such, there is a need to explore agricultural systems that promote forest and environmental conservation. Studies on decision support frameworks that can help farmers and landowners make informed optimal land use choices are limited in the country. This study attempts to fill the gap. We used survey questionnaire and focus group discussion to gather data from farmers and other stakeholders. Multi-Criteria Decision Analysis (MCDA) technique was employed to analyse the data. The results showed that agroforestry, with the highest ranking (probability of 0.98), is the optimal land use option. Coconut and oil palm land use options were perceived to be contributing to the degradation of the periphery of the Ankasa Conservation Area (ACA). Intensifying education and training will increase the uptake of agroforestry as an integrated multifunctional land use strategy in the ACA.

Keywords: agriculture; agroforestry; decision support system; Ghana; protected area.

read more

Cornhusk Powders as Adsorbents for Nitrites in Solution: A Thermodynamic and Kinetic Approach

Alina Elena Trofin, Elena Ungureanu, Iuliana Motrescu, Lucia Carmen Trincă, Denis Constantin Țopa, Diana Beatrice Eperjessy

ABSTRACT. The retention of nitrite ions in solutions of different concentrations by three cornhusks-based powders was analyzed. Natural cornhusk powder (NCHP), as waste obtained from local market, the alkalized cornhusk powder (ACHP) and the biochar from the original material (CHBC) have been characterized through scanning electron microscopy (SEM) and elemental composition EDAX – TEAM analysis (Energy dispersive analysis X-ray – Texture and ele-mental analytical microscopy) and tested for the removal of nitrite ions. The influence of initial nitrite concentration and contact time was studied under slow stirring rate conditions (150 rpm). For all three adsorbents both Freundlich and Langmuir isotherm equations described the process with R2 > 0.95, denoting physical adsorption and chemisorption on the surface. The estimated retained quantities (mg·g-1) determined from isotherms were 4.4783 (NCHP), 8.3542 (ACHP) and 8.7413 (CHBC). The Ho&McKay model was better adjusted to the adsorption data with R2 > 0.985, while the Lagergren model produced regression factors between 0.61 and 0.88. Considering the biggest concentration of nitrite solution of 50 mg·L-1 and the longest contact time of 150 minutes, the equilibrium capacity qe (mg·g-1) predicted by the Ho&McKay model for the considered adsorbents were: 4.5065 (NCHP), 8.5179 (ACHP) and 8.9445 (CHBC) compared to the obtained qt (mg·g-1) of 4.4384 (NCHP), 8.0685 (ACHP) and 8.5753 (CHBC). The nitrite uptake in the experiments reached a maximum of 2.2192 mg·g-1 on NCHP, 4.0342 mg·g-1 on ACHP and 4.2877 mg·g-1 on CHBC. Considering the cost-effective treatment steps, there is the possibility of valorising an important amount of waste as adsorbent materials.

Keywords: cornhusks powder; nitrite removal; waste valorisation.

read more

Impact of the COVID-19 Pandemic on Embryo Transfer Activities in Europe

Ștefan Gregore Ciornei, Dan Drugociu, Florin Nechifor, Petru Roșca

ABSTRACT. This review highlights to what extent the embryo transfer (ET) activity in Europe was affected by the COVID-19 pandemic. The ET represents a procedure of modern reproductive biotechnologies that is increasingly applied in farms animals. Commercial ET is applied to cattle and, albeit to a lesser extent, to other species. For this study, official data reported by each country to the European (AETE) and international (IETS) forums, regarding ET activities, were accessed. Each country has an official rapporteur, but the procedures are not mandatory. The reported data included the total number of ET sessions, the type of embryos obtained in vivo derived (IVD) or in vitro produced (IVP), the collection rate of oocytes and embryos and transferable embryos produced both in cattle and other species. For comparison, the data of two similar pre-pandemic (2017 and 2018) and pandemic (2019 and 2020) periods were analysed. The pandemic period (2019) started with a record decrease in all categories of ET, most likely because of less reports by veterinaries since reporting took place in May 2020, already pandemic time Europe, indicating a false negative decrease in Europe, the total ET activities reported decreased by 31.59%. In 2019, the in vivo ET procedures in cattle decreased by 26,421 sessions compared to 2018 (approximately 20%). Surprisingly, during the pandemic, in vivo ET in equines increased by almost 40%.

Keywords: covid; embryo transfer; IVD; IVP; pandemic.

read more

Phenotypic Variability Evaluation of Wheat Varieties from the Suceava Gene Bank Collection

Tiberiu Emilian Sârbu, Iulian Gabur, Dănuț Petru Simioniuc, Daniela Domnica Plăcintă, Danela Murariu, Violeta Simioniuc

ABSTRACT. The intensity of selection, inadvertent pathways of domestication, and the influence of climate change collectively amplify the frequency of detrimental alleles. This phenomenon, in turn, triggers genetic drift, leading to an in-advertent decline in the diversity of plant genetic resources. Genetic variability is of utmost importance for a species’ adaptability and overall fitness. Our investigation centres on Triticum germplasm within the agroclimatic conditions of the Suceava Gene Bank, encompassing 2021–2022 field trials. Comprehensive morphophysiological characterisation was conducted across 200 cultivated varieties, spanning three Triticum species (T. aestivum, T. turgidum and T. monococcum). Initially, the data underwent meticulous processing involving the computation of amplitude of variation, variance (s2), standard deviation (√s), and coefficients of variation (s%) for three pivotal agronomical traits: plant height, spikelets per spike, and total seeds per spike. Furthermore, an extensive cluster analysis was performed, encompassing multiple vital plant descriptors. The findings unveiled a remarkable dispersion of data, with standard deviation, amplitude of variation, and coefficient of variation collectively indicating substantial variability among the cultivated varieties. Within the same population, an intriguing observation emerged; of the 200 genotypes analysed, 83 exhibited immunity to Septoria tritici. Delving deeper into the statistical analysis, we identified two primary clusters within the population. Overall, a significant proportion of this germplasm showcased elevated phenotype scores, rendering them well-suited for further exploration as foundational material in pre-breeding initiatives.

Keywords: cluster analysis; morphophysiological traits; Septoria tritici; Triticum germplasm.

read more

Effect of Gliricidia sepium Leafy Biomass and NPK (15:15:15) Fertiliser on the Growth and Yield of Tomato, Solanum lycopersicum (L.)

Adams Latif Mohammed, Esther Korkor Nartey, Frank Addai, Samuel Arthur, Elvis Bawah

ABSTRACT. The use of organic amendments and appropriate nutrient management practices is essential for sustainable agricultural production. The present study investigated the effect of Gliricidia sepium leafy biomass and NPK (15:15:15) on the growth and yield of tomato plants (Solanum lycopersicum L.). A randomised complete block design with five treatments and four replicates was employed in this experiment. The treatments included T1 (Control), T2 (100% G. sepium leafy biomass), T3 (25% G. sepium leafy biomass + 75% NPK fertiliser), T4 (50% G. sepium leafy biomass + 50% NPK), T5 (100% NPK), and T6 (75% G. sepium leafy biomass + 25% NPK). The application of G. sepium leafy biomass and NPK had significant effects on tomato growth and yield (p˂0.05). The combined application of G. sepium leafy biomass and NPK (T4) and the sole application of G. sepium leafy biomass (T2) resulted in the highest plant height, stem girth, and fruit yield compared to the other treatments. Additionally, treatment with 75% Gliricidia leafy biomass and 25% NPK fertiliser (T6) resulted in a superior fruit weight compared to the other treatments. The application of G. sepium leafy biomass (T2) alone and NPK alone (T5) showed a significant improvement in plant growth parameters and fruit yield compared to the control (T1), although the effects were not as pronounced when combined with NPK (T4). The application of NPK fertiliser (T3) also positively influenced plant growth and yield, but the effects were lower compared to T2, T4, and T6. Further studies are warranted to optimise the application rates of G. sepium leafy biomass and NPK for different tomato cultivars and growing conditions.

Keywords: agroforestry tree species; crop nutrition; fertilisers; growth; tomato plant.

read more

Dynamics of Urban Landscape and Its Thermal Interactions with Selected Land Cover Types: A Case of Benin City, Nigeria

Oseyomon John Aigbokhan, Oludare Hakeem Adedeji, Abiodun Olusegun Oladoye, John Adebayo Oyedepo

ABSTRACT. In this study, the spatiotemporal dynamics of the urban environment and thermal environment of Benin City are analysed. The maximum likelihood algorithm for land use and land cover (LULC) analysis was used to categorise Landsat images. The relative transfer equation (RTE) and land surface emissivity (LSE) approaches were used to retrieve the land surface temperature (LST), whereas the Cellular Automata-Markov (CA-Markov) algorithm was used to forecast the LULC for 2030. The findings reveal evolving LULC patterns over time. Built-up areas made up 19.66% of the total area in 1990, bare ground made up 9.25%, and vegetation made up 71.08%. Built-up areas reached 23.40% in 2000, bare land reached 12%, and the vegetation cover dropped to 64.16%. In 2010, there was an increase in the proportion of built-up areas to 44.38%, the proportion of bare land increased to 22.20%, and the proportion of vegetation decreased to 33.42%. Built-up areas reached 61.79% in 2020, compared to 22.29% for bare land and 61.79% for vegetation. Regarding the relationship between the fractional vegetation cover (FVC) and LST, for the years 1992, 2002, 2012, and 2022, R2 is equal to 0.87097, 0.84598, 0.83957, and 0.71838, respectively. Conversely, for the LST and the normalised difference built-up index (NDBI), the R2 values were 0.5975, 0.73876, 0.86615, and 0.90368 for 1992, 2002, 2012, and 2022 respectively. In conclusion, this study evaluates Benin City’s metropolitan setting and thermal environment. According to the LULC study, there are more built-up areas and less vegetation. The impact of the changing land cover on urban thermal features is shown through correlation analysis, which links more built-up regions to higher LSTs. These results can support urban design efforts to lessen the effects of climate change. Examining the distribution of the LST and its associations with particular land cover types was the major goal of this study. Future research will undoubtedly use this study as a useful reference when modelling urban terrain and temperature variations.

Keywords: algorithm; concentric zone; emissivity; zonal statistics.

read more

Seaweed Culture, Post-Harvest Processing, and Market Generation for Employment of Coastal Poor Communities in Cox s Bazar

Asrafi Md. Farhaduzzaman, Md. Suzan Khan, Mohammad Hasan, Rashedul Islam, Mahadi Hasan Osman, Md. Neamul Hasan Shovon, Sayeed Mahmood Belal Haider, Mrityunjoy Kunda, Md. Tarikul Islam, Md. Simul Bhuyan

ABSTRACT. Seaweed farming is one of the aquaculture industries with the greatest growth rate worldwide, with an annual production of over 33 billion tonnes, and a value of USD$11.8 billion which is predicted to treble by 2024. The current study was conducted in the Bakkhali River estuary and set out to survey the seaweed cultivation, processing, marketing status of the seaweed farmers and evaluate its financial benefits. Gracilaria lemaneiformis and Ulva intestinalis were cultured using the rope methods. The yield of G. lemaneiformis and U. intestinalis was good (Ten 15 m long ropes were used in each of the 45 plots, produced 7770 kg of G. lemaneiformis in the first 15 days while using ten 15 m long ropes were in each of the plots of U. intestinalis produced 2300 kg). Water parameters viz. temperature, salinity, pH, DO and transparency were checked every 15 days. According to previous literature, winter was determined to be the most productive time for seaweed development in Bangladesh. Seaweed was processed by the sun drying method and sold to retail clients, neighborhood shops and markets, and representatives in Cox’s Bazar, Bandarban, Rangamati and Khagrachari district, where G. lemaneiformis attracting the higher price. The income of seaweed farmers has increased more than in the previous years to maintain their family. Most of the farmers used raw seaweed as traditional medicine for the treatment of gastric problem (80%), diabetes (5%), blood pressure (3%) and cancer (2%). They also used seaweed as an additive with other vegetables, mutton and chicken. Education for coastal people is very difficult, but after cultivating seaweed, about 60% of farmers can afford their children’s education cost and about 85% of the households had electric fans, while the majority had mobile phones. The cost-benefit analysis demonstrated that seaweed cultivation is highly profitable.

Keywords: coastal communities; employment generation; marketing; processing; seaweed culture.

read more

Prevalence of West Nile Virus Antibodies in Indoor Dogs From an Urban Area in Iași, Romania: Indicators of Viral Presence and Urban Transmission Potential

Luanda Elena Oșlobanu, Luciana Alexandra Crivei, Ioana Alexandra Rățoi, Ioana Cristina Crivei, Gheorghe Savuța

ABSTRACT. West Nile Virus (WNV), a zoonotic mosquito-borne virus (mobovirus) originally isolated from the blood of a febrile Ugandan woman in 1937, caused substantial human disease in Europe starting in the 1990s and emerged in 1999 in The United States of America (USA) for the first time. It has become an important concern for public health due to its reemergence and frequent human outbreaks. The enzootic transmission cycle of arboviruses involves primary wild animals; however, spillover transmission is reported frequently in domestic animals. Dogs are dead-end hosts in WNV transmission epidemiology. However, detecting WNV antibodies in the dog population can indicate the virus’s presence and spread in different areas. The virus is known to be endemic in parts of Romania, including Iași County. The study aimed at assessing the prevalence of anti-WNV antibodies in indoor dogs from an urban area in Iași, where all the conditions for virus transmission are met (wetland, density of wildlife hosts including birds, abundance of vectors, domestic mammal hosts and synanthropic birds). Using a commercial enzyme-linked immunosorbent assay (INGEZIM West Nile COMPAC, Ingenasa, Madrid, Spain), serum samples collected from indoor dogs between 2020–2022 were screened for WNV antibodies. The results showed an overall seroprevalence of 12.2%. Detection of specific antibodies in dogs suggests a possible establishment of an urban cycle for WNV or other antigenically related flaviviruses.

Keywords: dogs; seroprevalence; West Nile.

read more

The Influence of Climatic Conditions on the Occurrence of Large Forest Fires: A Case Study of Stara Planina Nature Park in 2007 and 2019

Stanimir Živanović, Milena Gocić

ABSTRACT. This work investigates the relationship between the occurrence of large forest fires (more than 1,000 ha) and meteorological data. Specifically, it analyzes the characteristics of large forest fires in July 2007 (2,500 ha) and October 2019 (2,108 ha) in Serbia’s Stara Planina Nature Park (NP). The results show that at these times, forest fires occurred during long heatwaves with low relative humidity and high air temperature. Dry climatic conditions in the preceding months also contributed to the occurrence of large forest fires, alongside the presence of wind. The study uses climate indices based on the combination of air temperature and precipitation. Forest drought index (FAI) data show that 2007 and 2019 were drier than the 1961–1990 climate period. According to the Lang Precipitation Factor Index (AILang), the periods June–July 2007 and July–October 2019 can be classified as dry. The De Martonne Drought Index (ImDM) shows that July 2007 and October 2019 had the characteristic of areism. These were also the periods when large wildfires were recorded. The results of this study could be used for wildfire risk assessment in protected areas and for fire prevention and suppression planning.

Keywords: climate indices; large forest fire; Serbia; Stara Planina Nature Park.

read more

The Influence of The Usual Holding Solutions on Hyacinthus orientalis Cut Flower Vase Life

Lucian Răus, Mariana Volf, Diana Elena Bolohan

ABSTRACT. In this study, four holding solutions very often used by florists and the final consumer were tested to analyse their impact on the development of the inflorescence, as well as the elongation, weight and degree of bending of the hyacinth flowers. The use of these solutions has a general character, and the flower species react differently due to both their genetics and the conditions in which they were cultivated, handled and stored. To verify the effectiveness of the preservative solutions on Hyacinthus orientalis vase life, four solutions were prepared with 2% sucrose and none or one of the follow-ing antimicrobial substances: sodium hypochlorite, acetic acid and ascorbic acid. During the study, measurements were made on the weight, elongation of the floral stems, chlorophyll content of the leaves and vase life days. The vase life of the hyacinths in this experiment was 5 days, except for the flowers from the 2% sucrose holding solution, which started to wilt on day 5. On day 6, the flowers showed signs of senescence. The increase in the length of the inflorescence stem showed significant differences for the sucrose + ascorbic acid holding solution, with a maximum of 0.8 cm on day 4. However, the interest was not to maximise the elongation of the floral stem but to slow down this process since elongation leads to the bending of the stem, causing it to require additional support.

Keywords: antimicrobial substances; holding solutions; inflorescence stem elongation.

read more

Bibliometric Analysis of the Incidence of Non-Covid Respiratory Disease under NPI Conditions During the Pandemic

Nji T. Ndeh, Yacob T. Tesfaldet, Oluseye Oludoye

ABSTRACT. Publications associated with the incidence of respiratory diseases in light of the COVID-19 pandemic have skyrocketed. Using bibliometric analysis, this study assessed the characteristics of published articles regarding non-pharmaceutical interventions (NPIs) on other respiratory infections during the COVID-19 pandemic. On July 06, 2023, a systematic review of literature on the incidence of respiratory diseases was conducted using the Scopus database. VOS viewer software was used to visualize the network. This study yielded a total of 910 articles, which were eventually narrowed down to 148 articles. The findings of the bibliometric analysis revealed a trend in research that included not only the investigation of the incidence of other respiratory diseases but also that of gastrointestinal infections during the COVID-19 pandemic. Most of the research output was created by American and Chinese authors. Kim J. H. was the most productive and cited researcher, with three research articles. There is a scarcity of data on the effectiveness of NPI in preventing the spread of other respiratory diseases among various age groups, including children, adults, and vulnerable populations. Countries should consider increasing their support for COVID-19 research, to generate knowledge pertaining to NPIs and, by extension, other infectious diseases with similar transmission mechanisms.

Keywords: bibliometrics; COVID-19; incidence; keyword analysis; nonpharmaceutical interventions; respiratory disease.

read more

Physiological and Biochemical Aspects of Optimising the Sowing Rate of Spring and Winter Pisum sativum Forms

Victor Shcherbakov, Vyacheslav Rudenko

ABSTRACT. The winter and spring varieties of peas (Pisum sativum) require careful study of the entire range of technological measures, including seeding rates, as the basis for optimal sowing density and the formation of phytocenose crops. This issue has not been resolved to date, not only in the context of agrobiological justification but also in everyday practice in Ukraine. This research was carried out in a field experiment in a three-way factorial experiment: factor “A” was the type of development – winter and spring; factor “B” was the variety – spring peas (Svit and Darunok Stepu) and winter peas (Moroz, Enduro and Baltrapp); and factor “C” was the seeding rate. According to the field studies, differences in the physiological and biochemical parameters of the P. sativum test culture characterised the photosynthetic activity of the plants, considering the type of development and variety. The winter varieties of P. sativum were characterised by a higher chlorophyll content (by 35–40%) compared to the spring varieties, which had an economic effect with an increase in the yield of dry biomass of the experimental crop and a decrease in seeding rates, with the formation and increase in grain yield by 14–18%. The intensity of chlorophyll in the process was not a determining factor in the accumulation of organic biomass. The extensive nature of the integration complex was noted (the amount of chlorophyll – the amount of biomass). At the optimal seeding rate, a certain specificity was observed in different types of P. sativum: for spring varieties, the sufficient rate was 0.9 million seeds/ha, and for winter varieties, it was 0.7 million seeds/ha. For varieties with a low productivity level (spring – Svit and winter – Moroz), the optimum sowing rate was around 0.7 million seeds/ha. The increase in the seeding rate was accompanied by a decrease in the content of various chlorophyll fractions from 10–12% to 20–26%.

Keywords: chlorophyll; economic effect; Pisum sativum; productivity; seeding rate; varieties and types of development.

read more