Journal of Applied Life Sciences and Environment (ALSE) is a peer-reviewed, open-access journal on Agricultural, Horticultural and Animal Sciences, Food Sciences, Veterinary Medicine and Environmental Management in the field of Applied Life Sciences, published quarterly online and in print by “Ion Ionescu de la Brad” Iasi University of Life Sciences (IULS).
Latest Articles
Predictive Air Pollution Assessment Using Matrix Algebra And GIS/GPS in Aguleri Anambra State
Leonard Chukwuemeka Anyika, Chidi Obi
ABSTRACT. This study assessed the air pollution loads of sulphur dioxide (SO2), nitrogen dioxide (NO2) and particulate matter (PM10) from Aguleri in Anambra State of Nigeria using matrix algebra and the geographical information system (GIS)/global positioning system (GPS) attachment to MATLAB. The pollutant values of SO2 and NO2 were obtained using the Crowcon Gas Monitor Model CE 89/336/EEC, while the PM10 values were obtained with the Crowcon Particulate Monitor Model No.1000 with the serial number 298621. The pollution characteristics of the study area were simulated using the polynomial expression yi = k + k1x1 + k2x2 + k3x3 +… knxn.. The predictive parameter constants, k, were determined with the solution to the simultaneous equations arising from the polynomial expressions using matrix algebra. MATLAB 7.9 curve fitting software was used to produce associated model equations from the fitted curves for the variations of SO2, NO2 and PM10 as a function of locations in Aguleri for both rainy and dry seasons. The evaluation of pollution models used for the study showed that constants from the fitted curves do not closely match constants from ab initio calculations. The corresponding coordinates in both GIS/GPS contour and surface plots revealed a pollution distribution concentration of 50% in Aguleri. The results revealed that the stations in Aguleri had a satisfactory air pollution index rating. This study serves as an improvement to air quality studies and a veritable tool for air quality management and policymaking.
Keywords: air pollutants; particulate matter; polynomial equations; seasons; software.
Sound and Silence: A Comparative Study of Noise Pollution in Shimla’s Residential and Commercial Sectors, Himachal Pradesh, India
Hritik Thakur, Pawan K. Attri
ABSTRACT. Noise pollution is a growing global issue, impacting both developed and developing countries, including India. Shimla, a historically significant city and a popular hill station in Himachal Pradesh, is experiencing increasing noise pollution due to its expanding population, more vehicles, congested roads, and tourists. This study evaluates noise pollution in Shimla’s commercial and residential areas, comparing current levels with Central Pollution Control Board (CPCB) standards and proposing mitigation measures. Noise was measured using a Metravi (SL-4010) sound level meter, with readings taken every three minutes for three hours in the morning, at noon, and in the evening. The equivalent continuous noise level (Leq) was calculated and compared to standards. Measurements were conducted in March and April 2024 at 15 locations. Additionally, a survey of 100 people assessed the causes and effects of noise pollution. Results showed that the Leq dB(A) values ranged from 76.87 dB(A) at Boileauganj Chowk to 84.97 dB(A) at the Old Bus Stand in commercial areas. In residential areas, Khalini Chowk recorded the highest Leq of 80.56 dB(A), while Mall Road had the lowest at 76.87 dB(A). Vehicles were identified as the primary noise source by 75% of respondents. In the survey, 51.67% of respondents reported irritation due to high noise levels, and 38.33% experienced headaches, highlighting the adverse effects on residents’ well-being and health. All locations exceeded CPCB standards for noise levels. The study recommends measures to reduce noise pollution in Shimla and suggests further comprehensive noise pollution studies in Himachal Pradesh.
Keywords: commercial area; noise pollution; residential area; shimla.
Assessing the Impact of Agricultural Practices on Environmental Sustainability in Nigeria
Bright Oluwatomilola Olunusi
ABSTRACT. This study investigates environmental sustainability and growth trends in Nigeria over a 30-year period (1991–2020), focusing on key indicators such as agriculture value added, forest area, employment in agriculture, employment in industry, and renewable energy consumption. Time series analysis reveals a linear increase in deforested land and agricultural expansion, alongside a decline in agricultural employment, which stabilised around 2013. Employment in the industry showed a dip until 2011, then increased steadily. Gender-disaggregated data highlights a notable disparity in agricultural employment, with significantly more males engaged compared to females. Using regression analysis with leaps, autoregressive distributed lag models, and Granger causality tests, the study identifies strong associations between deforested land and predictor variables such as agricultural land percentage, employment in industry, and renewable energy consumption. The results show that these variables significantly predict deforestation. Interestingly, a significant negative association was found between employment in agriculture and deforestation, although causality tests indicated no significant causation, suggesting a nuanced relationship influenced by factors like land-use conflicts and climate change. These findings highlight the nexus between socio-economic factors and environmental outcomes, emphasising the need for targeted policies to address deforestation, promote sustainable land management, and reduce gender disparities in agriculture. This research provides valuable insights for policymakers and stakeholders aiming to foster sustainable development and inclusive economic growth in Nigeria and across Africa.
Keywords: agricultural land-use change; deforestation; economic development; environmental sustainability; renewable energy; time series analysis.
Morphological and Physiological Responses of Maize to Varying Nitrogen Sources and Stress Levels in Hydroponic Systems: a Comparative Study
S.M. Abidur Rahman, Billal Hossain Momen, Rashedur Rahman Tanvir, Bitopi Biswas, Mosammat Nilufar Yasmin, Tariful Alam Khan, M. Robiul Islam
ABSTRACT. A hydroponic experiment was conducted at the Precision and Automated Agriculture Laboratory, Department of Agronomy and Agricultural Extension, University of Rajshahi, from April to August 2023. The objective was to investigate the morphological and physiological responses of maize to varying nitrogen sources and stress levels in hydroponic systems. The experiment comprised three nitrogen treatments: CN (100% chemical nitrogen as 2 mM NH4NO3), ON (100% organic nitrogen as 4 mM glycine), and LN (low nitrogen as 10% of 2 mM NH4NO3 chemical nitrogen solution). The popular maize variety NH7720 (marketed by Syngenta Bangladesh Limited) was used. The experiments followed a completely randomised design with three replications. The CN treatment consistently outperformed the ON and LN treatments in various growth-related parameters, including plant height (72.73 cm), leaf area (295.54 cm²), shoot dry weight (0.65 g/plant), total chlorophyll content (3.11 mg/g), and shoot (11.06%) and root (10.82%) protein content, indicating that adequate nitrogen treatment stimulated strong growth and development in maize plants. Conversely, the LN treatment exhibited a superior shoot-to-root ratio (85.43%), proline accumulation (188.01 µg/g), number of root tips (21.25), root length (31.65 cm), root network area (619.10 cm²), root diameter (5.63 mm), root volume (13944.71 mm³), and root surface area (3705.51 mm²). These results suggest that under nitrogen-deficient conditions, maize plants allocate resources to root development and stress tolerance mechanisms. The organic nitrogen (ON) treatment showed intermediate results, being statistically similar to both the CN and LN treatments across a range of characteristics, suggesting that organic nitrogen or glycine might be less effective than chemical nitrogen or ammonium nitrate in promoting optimal maize growth.
Keywords: hydroponic experiment; maize; morphological adaptation; nitrogen stress; physiological adaptation.
Yield and Quality Characteristics of Sunflower (Helianthus annuus L.) Cultivars by Different Sowing Dates
Mehmet Necat İZGİ, Abdullah ÇİL, Ayşe Nuran ÇİL
ABSTRACT. A field trial was initiated to ascertain the optimal sowing date for five distinct sunflower cultivars in Turkey’s Mardin Province. The trial scrutinised various parameters including plant height, head diameter, seed yield, protein content, oil content and oil yield. The first sowing produced the highest seed yields (3484 kg ha−1), with the Şems (3255 kg ha−1) and Zuhat (3157 kg ha−1) cultivars producing the highest values. The highest overall oil content was achieved in the second sowing (31.7%) and in the Zuhat cultivar (34.6%). The highest mean oil yields were observed in the Zuhat cultivar (1096 kg ha−1) and in the first sowing (1084 kg ha−1). There was a general decrease in yield as the sowing date was delayed. Winter sowing is suggested for the Zuhat cultivar. If possible, the second sunflower crop should be sown shortly following the harvest of the main crop.
Keywords: cultivar; oil content; protein content; sunflower; sowing date; yield.
Protein Content Stability of Triticale Genotypes Under Multiple Environments Using AMMI Analysis
Enver Kendal
ABSTRACT. Triticale is an important source of protein in animal nutrition, both as grain and silage. Protein is a quality criterion that is strongly affected by the environment and genetic factors. The objectives of this study were to assess genotype–environment (G-E) interactions and determine and compare stable genotypes to recommend for further evaluation. The protein content of 12 genotypes obtained from 7 environments were evaluated using additive main effect and multiplicative interaction (AMMI) analysis in the 2014–2015 and 2015–2016 growing seasons in 4 locations in Turkey. The variance of AMMI analysis based on protein content showed a significant effect of environment, genotype and G–E interaction, with a 93.0, 2.3 and 4.5% total variation, respectively. Partitioning of the total sum of squares showed that the effect of environment was a predominant source of variation, followed by the G–E interaction and genotype effect. AMMI analysis showed that the first principal component was highly significant at P < 0.01. The mean grain protein content varied from 14.9 to 16.2% among the genotypes and from 10.9 to 18.8% among the environments. AMMI analysis indicated that G3, G12 and G1 were quite promising genotypes. G8, which was used as a variety candidate in this research, was quite stable but had a low protein value. In addition, G3, G11 and G12 had a higher protein content than the standard varieties evaluated in this research. G3 had both a high protein content and stable genotype; therefore, it can be recommended to release as a candidate. As AMMI analysis indicated, E1 and E7 environments were especially suitable for protein studies in triticale, while E4, E5 and E6 showed poor results and were not suitable environments.
Keywords: AMMI; location; protein content; triticale.
Cover Story
Plastic debris pollution is one of the world’s most prevalent pollution problems, affecting oceans and inland waterways. An estimated 10% of plastic waste ends up in the ocean, endangering marine turtles and altering the physical properties of the marine environment.
As a result of mass production, plastics have spread throughout the marine environment and are now particularly common in marine ecosystems, significantly threatening environmental and human health as well as the economy. Most of this plastic waste comes from unregulated use, manufacturing and importation, indiscriminately dumped or deposited at dumpsites and landfills. Significant quantities of plastic debris originate from various activities such as tourism, fishing and other recreational activities on beaches.
If waste management does not improve, the total amount of plastic waste entering the ocean is expected to increase by an order of magnitude by 2025. Evidence suggests that even remote and protected areas are not immune to plastic pollution, and it is essential to identify marine macro-plastics that have washed up on beaches to determine the source of the pollution and develop the best cleanup plans. In the Philippines, for example, marine plastic research is critical because the country is heavily reliant on the marine environment and the ecosystem services it provides. Results of a macro-plastic waste characterisation study along the coastal boulevard to develop both ecologically and economically sound management plans and policy recommendations are presented on page 183.
Enhancing Maize Productivity with Infield Rainwater Harvesting Techniques and Cattle Manure in Semi-Arid Areas Of Zimbabwe
Andrew Tapiwa Kugedera, Ponesai Mutero, Letticia Kudzai Kokerai
ABSTRACT. Soil fertility and moisture management can be sustainable ways to improve crop production in low rainfall areas. The aim of this study was to evaluate the effects of infield rainwater harvesting and cattle manure on maize yield, rainwater use efficiency, agronomic efficiency, and the value–cost ratio. The experiment used a split plot design with three in situ rainwater harvesting (IRWH) techniques (planting pits, infiltration pits, and conventional tillage (as a control)) as the main treatment factor and cattle manure as the sub-plot factor at four levels (0, 2.5, 5, and 10 t ha−1). The interactive effects of IRWH, cattle manure, and season were significant among all parameters measured (p<0.05). The highest maize grain yield (3990 kg ha−1) was obtained from the planting pits with 10 t ha−1 cattle manure in the 2022/23 cropping season. Maize stover yield increased with an increase in cattle manure, with the highest yield of 6450 t ha−1 at 10 t ha−1 cattle manure. Rainwater use efficiency was significantly (p<0.05) increased by an average of 2.5 kg ha−1 mm−1 from 0 to 2.5 t ha−1. Agronomic use efficiency significantly decreased with the increasing application rate of cattle manure (p<0.05). The interaction of planting pits and 2.5 t ha−1 cattle manure had the highest cost ratio of 6.66 in the 2022/23 season. The interaction between planting pits and 10 t ha−1 cattle manure resulted in higher maize yields and rainwater use efficiency. However, it is recommended that smallholder farmers use planting pits and 2.5 t ha−1 cattle manure to obtain higher yield increments and high profits in high-risk climates. Keywords: agronomic efficiency; infiltration pits; maize production; planting pits; value–cost ratio.
Lead Levels in Milk and Its Products From Various Environments in Egypt
Assem Abou-Arab, Mahmoud Abou Donia, Ali Enb
ABSTRACT. Milk and its products are considered healthy due to their content of key nutritional elements. Despite their essential role in the human diet, they may be susceptible to contamination with many chemical pollutants from the surrounding environment, most notably heavy metals such as lead, which is considered toxic to consumers. This study evaluated lead concentrations in milk and dairy product samples collected from various Egyptian environments. The results showed significant differences (p < 0.05) in Pb concentrations in the samples collected from different environments in Egypt (industrial, traffic and rural). The average lead content in raw cow’s milk, sterilised milk, Domiati cheese, Ras cheese, processed cheese and yoghurt samples from industrial areas were 2.23, 0.33, 3.19, 5.10, 0.11 and 0.09 mg/kg, respectively. In traffic areas, lead concentrations were 1.83, 0.30, 2.72, 4.72, 0.13 and 0.09, and in rural areas, they were 0.61, 0.27, 1.52, 3.13, 0.13 and 0.09. The data showed that Pb levels in dairy product samples collected from industrial areas are very high compared with those collected from traffic areas. By contrast, the lowest Pb concentrations were recorded in samples from rural areas. It is recommended that lead levels in different foods, especially milk and its products, be evaluated at regular intervals. Keywords: dairy products; environments; lead; milk.
The Impact of Climate Change on The Sustainability of Wine Production and the Structure of Wine Consumption in the Northeast Region of Romania
Gabriela Ignat, Bianca Antoanela Ungureanu, Ioan Prigoreanu, George Ungureanu
ABSTRACT. This study was carried out with the aim of investigating and analysing different aspects related to viticulture and wine production in the northeast region of Romania. The methodology focused on the use of statistical data and field research, with an emphasis on the impact of potential climate change on wine production and consumer preferences. The analysis of the evolution of vine cultivation in the northeast region of Romania was carried out using statistical data on grape harvest, area planted to vines and wine production. The results showed significant changes in these indicators over the period analysed (2013-2022), reflecting the dynamics of the wine industry in the region. A survey of a sample of 50 respondents provided relevant data on their preferences. Analysis of this data revealed information on preferred wine types, consumption habits and purchasing preferences. Although the population‘s preference for white wine is evident, climate change may pose a challenge for the region, and in the future, it may be necessary to adapt vineyard locations and cultivate vine varieties more suitable for red wine production. These issues have important implications for planning and developing the future of the wine industry in the northeast region.
Keywords: climate change; distribution; production; sustainability; wine.
Effect of Dairy and Meat Wastewater Irrigation on Seedling Growth
Laura Carmen Apostol, Eufrozina Albu, Cristina Ghinea
ABSTRACT. Milk and meat processing industries release large quantities of nutrient-rich wastewater with organic compounds (proteins, fats, carbohydrates) in high concentrations. Reusing and recycling this biodegradable wastewater for crop irrigation could be a sustainable solution once the phytotoxic effects of wastewater on crops have been investigated. Therefore, the aim of this study was to evaluate the effects of milk and meat processing wastewaters on germination percentage, seed vigour indices, the seedling tolerance index and the phytotoxicity index of pea (Pisum sativum L.), sugar maize (Zea mays saccharata), purslane (Portulaca oleracea), wheat (Triticum aestivum) and red spinach (Amaranthus dubius). The two wastewater types were collected at the inlet of the city’s sewage system and analysed to determine their physicochemical and microbiological characteristics. The seeds of all five plant species were irrigated with untreated wastewater effluents. The highest – germination percentages were obtained for wheat (92%) and the lowest for red spinach (2.5%). Wheat, purslane, pea and red spinach samples irrigated with meat processing wastewater had higher germination percentages than samples irrigated with dairy wastewater. A higher phytotoxicity was observed for sugar maize, followed by red spinach irrigated with both types of wastewater. Future investigation into the effects of effluent dilution on these types of plants is recommended.
Keywords: food industry wastewater; phytotoxicity; seed germination; sugar maize; wheat.
Optimal Conditions for Extracting High-Quality Pectin From Water Hyacinth (Eichhornia Crassipes) for Less Soluble Hydrogel Production
Erawan Baothong, Pisit Chareonsudjai
ABSTRACT. The research aimed to extract high-quality pectin from water hyacinth for less soluble hydrogel production. The product adds value to water hyacinth waste and helps solve environmental problems. The high degree of esterification pectin can be prepared as a hydrogel, which can be used in various pollution treatment applications. The quality of pectin depended on raw materials and extraction conditions. The optimum condition was initially predicted using the response surface method (RSM). Three extraction variables were studied, including pH 1.0-4.0, temperature 50-110ºC, and extraction time 30-240 min. A total of seventeen runs including five replicate runs were studied. Functional groups of pectin were studied using Fourier Transform Infrared spectrophotometry. The degree of esterification and emulsifying properties were determined for assessing the quality of extracted pectin. The result revealed that the extraction conditions strongly affected both the yield and the quality. The condition of pH 2.5, 110ºC, and 30 minutes had the highest degree of esterification of 94.13 % but low yield (1.42%). On the other hand, under the conditions of pH 1.0, 110ºC, and 135 min extract time had the highest yield of 3.85% (about 76.6% of pectin content) however the degree of esterification is low at 43.25 %. Two mathematical models were proposed for yield and number of ester groups. The result will be used for the selection of high-quality pectin to produce insoluble hydrogel for pollution treatment in the future.
Keywords: Eichhornia crassipes; high-quality pectin; optimum condition; water hyacinth.
Effect of Pre-Exposure on the Insecticidal and Repellency Properties of Citrus paradisi Peel Essential Oil Against Tribolium castaneum
Adeyemi O. Akinyemi, Samuel A. Babarinde, Habeeb O. Adetunji, Oladeji K. Alalade
ABSTRACT. Although the use of essential oils (EOs) for pest control has gained popularity due to their biodegradability and ecological safety, a major concern regarding their use is stability. Therefore, this aspect deserves empirical studies to enable potential end users to adopt research findings on the pesticidal potential of EOs. This research investigated the insecticidal and repellence impacts of Soxhlet-extracted grapefruit peel (Citrus paradisi Macfad. (Rutaceae) Lane) EO pre-exposed to air for 0, 0.5, 1 and 2 h (before introducing insects) on Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). The ethanol diluents were initially allowed to evaporate for 15 min in all treatments. Contact toxicity was tested on larval and adult stages at 5 and 15% concentrations of the EO, while repellency was tested at a 5% concentration. The impacts of EO on adult behavioural activity were also observed 24 h after exposure. Grapefruit EO was effective in controlling T. castaneum adults and larvae at higher doses. Exposure periods had an immediate significant effect on larvae and adult mortalities 1 and 6 h after the insects were introduced. At a 15% concentration, EO with a 0 h pre-exposure period had significantly higher adult and larva mortalities after 6 h than those of 0.5, 1 and 2 h pre-exposure. Regardless of oil pre-exposure, insects were repelled, especially in test periods between 12 and 30 min. Therefore, grapefruit EO could be formulated for increased stability when an immediate impact is needed.
Keywords: exposure periods; grapefruit peel; mortality; pest control; toxicity.
Response of Sweet Corn Varieties to Plant Density and Tiller Removal: Preliminary Studies
Răzvan Huțuliac, Cristina Precupeanu, Ionela Cătălina Vasilachi, Alexandru Cojocaru, Mihaela Roșca, Vasile Stoleru
ABSTRACT. The sweet corn breeding strategy aims to enhance and sustain a consistent yield over time. The interaction between density and tiller removal is currently being discussed among sweet corn farmers, as well as scientists working in sweet corn. Considering the studies carried out to date and the research directions suggested by them, the present study aimed to determine the impact of plant density, variety, tiller removal and their interactions on the yield and ear prolificacy/weight of sweet corn under the climatic conditions of northeast Romania. The experiments were focused on 3 sweet corn varieties (Deliciosul de Bacău, Deliciul verii and Royalty F1), sown at 3 densities (50k, 60k and 70k plants/ha) and considering 2 variants for tiller removal (tiller removal / without tiller removal). The highest yield of sweet corn was recorded in the Royalty F1 hybrid in the plots in which the tillers were removed (24.27 t/ha). The Royalty F1 hybrid also had the heaviest ears (313.33 g) in plots with tiller removal and 60k plants/ha. Overall, the most favourable plant density in terms of yield was 60k plants/ha. The experimental results showed that in the climatic conditions of northeast Romania, tiller removal led to increased yield, especially in the Deliciosul de Bacău hybrid. Tiller removal in this hybrid resulted in a significant increase in yield (from 18.41 to 23.08 t/ha).
Keywords: plant density; sweet corn variety; tillers impact; yield.
A New Natural Food Dye: Microencapsulated Cornelian Cherry Bioactive Compounds
Iuliana-Maria Enache, Liliana Lucescu Ciurlă, Nicoleta Stănciuc, Antoanela Patraș, Camelia Vizireanu, Liviu Mihai Irimia
ABSTRACT. Cornus mas (CM) is one of the four edible fruits of the Cornus genus, a rich source of biologically active compounds (BACs) such as vitamins (like vitamin C), carotenoids, iridoids, and phenolics (phenolic acids, anthocyanins, and other flavonoids). This study aimed to analyse the improvement of the stability of CM anthocyanins by microencapsulation, in order to propose a new natural food dye. Microencapsulation using a mixture of whey protein isolate (WPI) and chitosan (CH) as wall materials has been applied to protect anthocyanins against external factors (e.g., light, temperature, storage, etc.). Two experimental variants of microencapsulated powders, WPI:CH = 1:1 (CH1) and WPI:CH = 1:2 (CH2), were realised by varying the wall materials ratio. The cornelian cherry fruit concentrated extract was evaluated for its phytochemical, colourimetric, and antioxidant capacities. Due to the excellent anthocyanin encapsulation effectiveness (74.29 – 88.71%), the wall materials utilised for both powders can be considered effective choices to safeguard the anthocyanins. All tests performed on the microencapsulated powders demonstrated that both suggested experimental forms can serve as a healthy substitute for artificial food additives. The incorporation of cornelian cherry fruit extract and microencapsulated powders into a food matrix (jelly candies) allowed examination of their effectiveness. The colour analysis rigorously characterised all the colour parameters related to red nuances (due to anthocyanins content, such as cyanidin-3-glucoside) and yellow nuances (associated with carotenoids content).
Keywords: chitosan; jelly candies; natural pigments; phenolic compounds; whey protein isolate.
Effect of Okoro [(Albizia zygia (J.F.) Macbr)] Leafy Biomass and NPK Fertiliser on the Growth and Yield of Sweet Pepper (Capsicum annum L.)
Adams Latif Mohammed
ABSTRACT. Agricultural productivity and sustainable crop management are pivotal aspects of global food security and economic stability. The quest for efficient and environmentally friendly practices in crop production has led to a surge in research exploring the utilisation of organic materials alongside conventional fertilisers to enhance crop growth and yield. An experiment was conducted over a growing period of three months, starting in August 2023 at the research field of Kwame Nkrumah University of Science and Technology, Kumasi-Ghana, to investigate the effects of Albizia zygia leafy biomass in combination with NPK fertiliser on the growth and yield of sweet pepper (Capsicum annum L.). A randomised complete block design was employed with four treatment groups replicated four times, including various combinations of A. zygia leafy biomass and NPK fertiliser, alongside control groups of sole fertiliser application and untreated plots (T1). The treatments were as follows: T1 = No biomass, no NPK (control); T2 = 0.5348 kg of A. zygia leafy biomass per 4.86 m2 (1100.5 kg/ha of A. zygia leafy biomass); T3 = 0.2463 kg of NPK per 4.86 m2 (506.7 kg/ha of NPK); and T4 = 0.2674 kg of A. zygia leafy biomass per 4.86 m2 + 0.1231 kg of NPK (550.3 kg/ha of A. zygia leafy biomass + 253.4 kg/ha of NPK). The assessed parameters were plant height, number of leaves, number of branches, fruit yield and fresh fruit weight. Significant variations (p ˂ 0.05) were observed in the growth parameters (plant height, number of leaves, number of branches) and yield attributes (fruit yield and fresh fruit weight) of sweet pepper among the treatments. The combined application of A. zygia leafy biomass and NPK fertiliser (T4) exhibited increased effects on plant height, number of leaves, number of branches, fruit yield and fresh fruit weight compared to individual treatments and control groups (p ˂ 0.05). Notably, the treatment combining A. zygia leafy biomass and NPK fertiliser (T4) demonstrated a substantial increase in plant height (44.58 cm), a significant improvement in the number of leaves (42.98) and a remarkable enhancement in the number of branches (11.33) compared to the sole applications and the control group. Furthermore, the combined treatment significantly increased (p ˂ 0.05) the yield parameters, including fruit yield and fresh fruit weight per hectare, showcasing an increase of 8796/ha and 23.91 t/ha, respectively, compared to sole fertiliser application and the control. The findings from this study demonstrated that the incorporation of A. zygia leafy biomass, either solely or in combination with NPK fertiliser, positively impacted the growth and yield of sweet pepper plants. These results highlight the potential of A. zygia leafy biomass as a valuable organic amendment for sustainable and enhanced crop production, offering promising implications for agricultural practices aimed at improving yield and plant growth.
Keywords: Albizia zygia; Capsicum annum (L.); NPK fertiliser; organic amendment; sweet pepper.
Characterisation of Macro-Plastic Waste Along The Philippines Longest Coastal Boulevard: Basis for Solid Waste Management and Policy Formulation
Lucy O. Elep Jr., Maria Cristina C. Azuelo
ABSTRACT. This study focused on the characterisation of macro-plastic waste in terms of types and quantities along the Philippines’ longest coastal boulevard, considering density and cleanliness as a basis for solid waste management and policy formulation. Macro-plastic identification and counting were conducted in four (4) municipalities and eight (8) barangays using the standing stock survey method. There were six (6) types of macro-plastic waste, with the highest quantity consisting of beverage (37.2%) and food packaging (30.5%), followed by fishing gear (17.0%), plastic utensils (9.2%), toiletries (4.8%) and household wastes (1.4%). The computed overall density from a total of 3,978 macro-plastic waste items over 12,000 m2 of beach area sampled was 0.3 (CM), meaning there were 0.3 litter items of plastic per m2 throughout the whole boulevard. The computed overall beach cleanliness was 6.6, which means that the cleanliness status is moderate. Overall, most macro-plastic waste is generated by locals or visitors. In conclusion, a policy regulating the use, littering, and carrying of plastic along boulevards is recommended to prevent plastic pollution considering the province’s growing ecotourism and the future expansion of the coastal boulevard.
Keywords: coastal road; ecotourism; social plastic.
Cover Story
Jellyfish (JF) are essential to marine ecosystems. However, JF that develop rapidly can have negative effects. On 3–4 August, 2022, a significant JF (Lobonemoides robustus Stiasny, 1920) bloom was observed along the Cox’s Bazar coast in Bangladesh (from Najdirartek to Sabrang). The goal of the current investigation was to identify the fatty acids (FAs) and amino acids (AAs) of L. robustus. The AAs were determined using liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis, while the FAs were determined using a gas chromatographic system with a flame ionisation detector. The most prevalent AA was glycine. The most common FA was linoleic acid (C18:3) (0.43%), followed by myristic acid (0.12%), cis-9-oleic acid (0.18%), gamma-linolenic acid (0.24%), and heptadecanoic acid (0.29%). Based on its high AA and FA contents, L. robustus is a great candidate for the potentially sustainable manufacture of nutraceutical, cosmeceutical, and biomedical natural products to improve health and well-being. In addition, the edible L. robustus could be exported to other countries for use as the bloom species, thus playing a major role in achieving a blue economy (see page 107).
Extraction and Characterisation of Natural Dye From Orange Peel for Textile Applications
Usman Habu Taura, Muhammad Abbagoni Abubakar, Abdulhalim Musa Abubakar, Mohammed Umar Kurgiya
ABSTRACT. Awareness of the need to protect the environment and people’s health has led to an intensification of concerns for obtaining sustainable products and processes. Toxic waste created during the production and use of synthetic dyes has an impact on both human and environmental health. As a result, natural dyes are more secure and safer than synthetic dyes. This study is significant because it has the potential to help develop sustainable and environmentally friendly textile dyeing techniques. In this study, a natural dye was successfully extracted from orange peel (Citrus recticulata Rutaceae) which was applied on textile fibre and was found to be partly effective for eco-friendly dyeing applications. The extracted dye does not have a good wash fastness, which is the ability of the fabric to retain its colour after washing. This weakness is demonstrated by carrying out a Fourier transform infrared (FTIR) analysis where the potential binding mechanisms between the dye and the textile fibres was revealed. Different functional groups can interact with the fibre’s functional groups, affecting the dye’s affinity for the fabric and its overall colour fastness properties. Findings show that dyes from orange peels showed promise in this study, but need to be improved further. Therefore, the study suggests that further research is needed to optimize the dyeing process and improve the fabric’s resistance to washing and other environmental factors.
Keywords: mordant; natural dye; orange peel; synthetic dye; textile industry.
Correction: Usman Habu TAURA’s affiliation has been changed from Department of Chemical Engineering, University of Maiduguri, P. M.B 1069 Maiduguri, Borno State, Nigeria in Oil and Gas Research Centre, Sultan Qaboos University, Muscat, Oman, 123, according to supporting document Journal of Applied Life Sciences and Environment_affiliation correction justification
Assessment of Sensitivity to Desertification in the Oum Er-Rbia Watershed (Upstream of Ouled Sidi Driss) Using The Medalus Approach
Souad Ourabit, Abderrahim Ettaqy, Mohamed El Ghachi
ABSTRACT. The issue of desertification is a pressing concern for many vulnerable regions, with consequences that extend far beyond their borders. It is a silent force that not only contributes to global climate change by releasing stored carbon from vegetation and soil but also compounds the ongoing environmental challenges we face on a global scale. In Morocco, the effects of these climate changes are already noticeable, particularly in terms of water scarcity due to reduced rainfall and rising temperatures. This, in turn, leads to soil drying and an increased risk of degradation. The Oum Er-Rbia watershed (upstream of Ouled Sidi Driss) is one of the basins affected by this issue, covering an area of 11,152 km² and spanning three topographic units (the Middle Atlas Mountains, the Phosphate Plateau and the Tadla Plain). This topographic diversity causes regional variation in the sensitivity to desertification. Therefore, this study aims to evaluate the sensitivity to desertification in this watershed by using the MEDALUS model (Mediterranean desertification and land use), which calculates the Desertification Sensitivity Index (DSI) through the composition of four indices: the Soil Quality Index (SQI), the Vegetation Quality Index (VQI), the Climate Quality Index (CQI) and the Land Use Quality Index (LUQI). The results show that the critical and highly sensitive zones represent 44% and are located at the northeast and west ends of the watershed. By contrast, the unaffected zones represent 12% of the total area of the watershed and correspond to the upstream area, where the climate, vegetation and land use systems are of good quality, providing effective protection for the land. To reduce the impact of this phenomenon, we have proposed some potentially impactful development actions based on land use and the results obtained.
Keywords: climate change; desertification sensitivity; drought; MEDALUS; Oum Er-Rbia watershed.
Growth Performance, Nitrogen Utilization and Economics of Red Sokoto Bucks Fed Saccharomyces Cerevisiae-Treated Groundnut Haulm and Dried Cassava Peel-Based Diets
Ahmad Rufai Musa, Olayinka John Makinde, Aminu Maidala, Ahmed Bishir, Kakudi Ismail Abubakar, Kosoro Ahmad Mua’zu, Ismaila Habiba Abdullahi, Yakubu Zakari Idrissa, Charles Hannatu, Muhammad Hamisu Zango
ABSTRACT. This study investigated the growth performance, nitrogen balance, and economics of production of Red Sokoto bucks (RSBs) fed Saccharomyces cerevisiae-treated, biodegradable groundnut haulms (SCGH) and dried cassava peels (DCP). Four dietary treatments were formulated including a control diet (T1; 0% SCGH:DCP) and DCP in combination with SCGH at the different proportions of 275:695 (T2), 375:595 (T3), and 475:495 (T4) g/kg of dry matter (DM). A combination of 5 g and 50 g of yeast as a probiotic and molasses, respectively, were added to 1 kg of GH after been dissolved in 1 L of water. Twelve healthy RSBs weighing 9.5 ± 0.40 kg of body weight (BW) and aged 7 to 8 months were exposed to four treatments. This was replicated three times in a completely randomized design (CRD). The experiment lasted for 90 days. The average final weight, average daily weight gain (gram/day), nitrogen intake, and nitrogen balance were significantly influenced by the inclusion of DCP and SCGH in the diets. There was no significant difference (p > 0.05) between the ADG of T1 (58.20 g/d) and the T4 (58.89 g/d) groups. Feed conversion ratio and feed-cost saving ($630.80) increased with the inclusion of DCP and SCGH in the goats’ diets. In conclusion, T4 (SCGH: 475g, DCP: 495g) had a better chemical composition, ADG, nitrogen balance, and feed-cost saving; therefore, SCGH are recommended and are readily available agricultural wastes that can be fed to RSBs to improve performance and nitrogen utilization in a cost-effective way, especially during dry season.
Keywords: bucks; cassava peels; nitrogen balance; probiotics; yeast.
First Report of The Amino Acid And Fatty Acid Composition of Jellyfish (Lobonemoides Robustus Stiasny, 1920) Collected During Jellyfish Bloom Along the Cox’s Bazar Coast, Bangladesh
Tarikul Islam, Simul Bhuyan, Mala Khan, Mrityunjoy Kunda, Sumi Akter, Nayan Kumer Kundu
ABSTRACT. Jellyfish (JF) are essential to marine ecosystems. However, JF that increases rapidly can have negative effects. On 3-4 August 2022, a significant JF (Lobonemoides robustus Stiasny, 1920) bloom was observed along Cox’s Bazar coast (from Najdirartek to Sabrang) in Bangladesh. The goal of the current investigation was to identify the fatty acids (FAs) and amino acids (AAs) of L. robustus. The AAs were determined using liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis, while the FAs were determined using a gas chromatographic system with a flame ionisation detector. The most prevalent AA was glycine. The most common FA was linoleic acid (C18:3) (0.43%), followed by myristic acid (0.12%), cis-9-oleic acid (0.18%), gamma-linolenic acid (0.24%), and heptadecanoic acid (0.29%). Based on its AA and FA contents, L. robustus can be a great candidate for the potentially sustainable manufacture of nutraceutical, cosmeceutical, and biomedical natural products to improve health and well-being. In addition, the edible L. robustus could be exported to other countries, thus way it can play a major role in achieving a blue economy.
Keywords: amino acids; bloom; blue economy; fatty acids; jellyfish.
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Cover Story
Plastic debris pollution is one of the world’s most prevalent pollution problems, affecting oceans and inland waterways. An estimated 10% of plastic waste ends up in the ocean, endangering marine turtles and altering the physical properties of the marine environment.
As a result of mass production, plastics have spread throughout the marine environment and are now particularly common in marine ecosystems, significantly threatening environmental and human health as well as the economy. Most of this plastic waste comes from unregulated use, manufacturing and importation, indiscriminately dumped or deposited at dumpsites and landfills. Significant quantities of plastic debris originate from various activities such as tourism, fishing and other recreational activities on beaches.
If waste management does not improve, the total amount of plastic waste entering the ocean is expected to increase by an order of magnitude by 2025. Evidence suggests that even remote and protected areas are not immune to plastic pollution, and it is essential to identify marine macro-plastics that have washed up on beaches to determine the source of the pollution and develop the best cleanup plans. In the Philippines, for example, marine plastic research is critical because the country is heavily reliant on the marine environment and the ecosystem services it provides. Results of a macro-plastic waste characterisation study along the coastal boulevard to develop both ecologically and economically sound management plans and policy recommendations are presented on page 183.
Enhancing Maize Productivity with Infield Rainwater Harvesting Techniques and Cattle Manure in Semi-Arid Areas Of Zimbabwe
Andrew Tapiwa Kugedera, Ponesai Mutero, Letticia Kudzai Kokerai
ABSTRACT. Soil fertility and moisture management can be sustainable ways to improve crop production in low rainfall areas. The aim of this study was to evaluate the effects of infield rainwater harvesting and cattle manure on maize yield, rainwater use efficiency, agronomic efficiency, and the value–cost ratio. The experiment used a split plot design with three in situ rainwater harvesting (IRWH) techniques (planting pits, infiltration pits, and conventional tillage (as a control)) as the main treatment factor and cattle manure as the sub-plot factor at four levels (0, 2.5, 5, and 10 t ha−1). The interactive effects of IRWH, cattle manure, and season were significant among all parameters measured (p<0.05). The highest maize grain yield (3990 kg ha−1) was obtained from the planting pits with 10 t ha−1 cattle manure in the 2022/23 cropping season. Maize stover yield increased with an increase in cattle manure, with the highest yield of 6450 t ha−1 at 10 t ha−1 cattle manure. Rainwater use efficiency was significantly (p<0.05) increased by an average of 2.5 kg ha−1 mm−1 from 0 to 2.5 t ha−1. Agronomic use efficiency significantly decreased with the increasing application rate of cattle manure (p<0.05). The interaction of planting pits and 2.5 t ha−1 cattle manure had the highest cost ratio of 6.66 in the 2022/23 season. The interaction between planting pits and 10 t ha−1 cattle manure resulted in higher maize yields and rainwater use efficiency. However, it is recommended that smallholder farmers use planting pits and 2.5 t ha−1 cattle manure to obtain higher yield increments and high profits in high-risk climates. Keywords: agronomic efficiency; infiltration pits; maize production; planting pits; value–cost ratio.
Lead Levels in Milk and Its Products From Various Environments in Egypt
Assem Abou-Arab, Mahmoud Abou Donia, Ali Enb
ABSTRACT. Milk and its products are considered healthy due to their content of key nutritional elements. Despite their essential role in the human diet, they may be susceptible to contamination with many chemical pollutants from the surrounding environment, most notably heavy metals such as lead, which is considered toxic to consumers. This study evaluated lead concentrations in milk and dairy product samples collected from various Egyptian environments. The results showed significant differences (p < 0.05) in Pb concentrations in the samples collected from different environments in Egypt (industrial, traffic and rural). The average lead content in raw cow’s milk, sterilised milk, Domiati cheese, Ras cheese, processed cheese and yoghurt samples from industrial areas were 2.23, 0.33, 3.19, 5.10, 0.11 and 0.09 mg/kg, respectively. In traffic areas, lead concentrations were 1.83, 0.30, 2.72, 4.72, 0.13 and 0.09, and in rural areas, they were 0.61, 0.27, 1.52, 3.13, 0.13 and 0.09. The data showed that Pb levels in dairy product samples collected from industrial areas are very high compared with those collected from traffic areas. By contrast, the lowest Pb concentrations were recorded in samples from rural areas. It is recommended that lead levels in different foods, especially milk and its products, be evaluated at regular intervals. Keywords: dairy products; environments; lead; milk.
The Impact of Climate Change on The Sustainability of Wine Production and the Structure of Wine Consumption in the Northeast Region of Romania
Gabriela Ignat, Bianca Antoanela Ungureanu, Ioan Prigoreanu, George Ungureanu
ABSTRACT. This study was carried out with the aim of investigating and analysing different aspects related to viticulture and wine production in the northeast region of Romania. The methodology focused on the use of statistical data and field research, with an emphasis on the impact of potential climate change on wine production and consumer preferences. The analysis of the evolution of vine cultivation in the northeast region of Romania was carried out using statistical data on grape harvest, area planted to vines and wine production. The results showed significant changes in these indicators over the period analysed (2013-2022), reflecting the dynamics of the wine industry in the region. A survey of a sample of 50 respondents provided relevant data on their preferences. Analysis of this data revealed information on preferred wine types, consumption habits and purchasing preferences. Although the population‘s preference for white wine is evident, climate change may pose a challenge for the region, and in the future, it may be necessary to adapt vineyard locations and cultivate vine varieties more suitable for red wine production. These issues have important implications for planning and developing the future of the wine industry in the northeast region.
Keywords: climate change; distribution; production; sustainability; wine.
Effect of Dairy and Meat Wastewater Irrigation on Seedling Growth
Laura Carmen Apostol, Eufrozina Albu, Cristina Ghinea
ABSTRACT. Milk and meat processing industries release large quantities of nutrient-rich wastewater with organic compounds (proteins, fats, carbohydrates) in high concentrations. Reusing and recycling this biodegradable wastewater for crop irrigation could be a sustainable solution once the phytotoxic effects of wastewater on crops have been investigated. Therefore, the aim of this study was to evaluate the effects of milk and meat processing wastewaters on germination percentage, seed vigour indices, the seedling tolerance index and the phytotoxicity index of pea (Pisum sativum L.), sugar maize (Zea mays saccharata), purslane (Portulaca oleracea), wheat (Triticum aestivum) and red spinach (Amaranthus dubius). The two wastewater types were collected at the inlet of the city’s sewage system and analysed to determine their physicochemical and microbiological characteristics. The seeds of all five plant species were irrigated with untreated wastewater effluents. The highest – germination percentages were obtained for wheat (92%) and the lowest for red spinach (2.5%). Wheat, purslane, pea and red spinach samples irrigated with meat processing wastewater had higher germination percentages than samples irrigated with dairy wastewater. A higher phytotoxicity was observed for sugar maize, followed by red spinach irrigated with both types of wastewater. Future investigation into the effects of effluent dilution on these types of plants is recommended.
Keywords: food industry wastewater; phytotoxicity; seed germination; sugar maize; wheat.
Optimal Conditions for Extracting High-Quality Pectin From Water Hyacinth (Eichhornia Crassipes) for Less Soluble Hydrogel Production
Erawan Baothong, Pisit Chareonsudjai
ABSTRACT. The research aimed to extract high-quality pectin from water hyacinth for less soluble hydrogel production. The product adds value to water hyacinth waste and helps solve environmental problems. The high degree of esterification pectin can be prepared as a hydrogel, which can be used in various pollution treatment applications. The quality of pectin depended on raw materials and extraction conditions. The optimum condition was initially predicted using the response surface method (RSM). Three extraction variables were studied, including pH 1.0-4.0, temperature 50-110ºC, and extraction time 30-240 min. A total of seventeen runs including five replicate runs were studied. Functional groups of pectin were studied using Fourier Transform Infrared spectrophotometry. The degree of esterification and emulsifying properties were determined for assessing the quality of extracted pectin. The result revealed that the extraction conditions strongly affected both the yield and the quality. The condition of pH 2.5, 110ºC, and 30 minutes had the highest degree of esterification of 94.13 % but low yield (1.42%). On the other hand, under the conditions of pH 1.0, 110ºC, and 135 min extract time had the highest yield of 3.85% (about 76.6% of pectin content) however the degree of esterification is low at 43.25 %. Two mathematical models were proposed for yield and number of ester groups. The result will be used for the selection of high-quality pectin to produce insoluble hydrogel for pollution treatment in the future.
Keywords: Eichhornia crassipes; high-quality pectin; optimum condition; water hyacinth.
Effect of Pre-Exposure on the Insecticidal and Repellency Properties of Citrus paradisi Peel Essential Oil Against Tribolium castaneum
Adeyemi O. Akinyemi, Samuel A. Babarinde, Habeeb O. Adetunji, Oladeji K. Alalade
ABSTRACT. Although the use of essential oils (EOs) for pest control has gained popularity due to their biodegradability and ecological safety, a major concern regarding their use is stability. Therefore, this aspect deserves empirical studies to enable potential end users to adopt research findings on the pesticidal potential of EOs. This research investigated the insecticidal and repellence impacts of Soxhlet-extracted grapefruit peel (Citrus paradisi Macfad. (Rutaceae) Lane) EO pre-exposed to air for 0, 0.5, 1 and 2 h (before introducing insects) on Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). The ethanol diluents were initially allowed to evaporate for 15 min in all treatments. Contact toxicity was tested on larval and adult stages at 5 and 15% concentrations of the EO, while repellency was tested at a 5% concentration. The impacts of EO on adult behavioural activity were also observed 24 h after exposure. Grapefruit EO was effective in controlling T. castaneum adults and larvae at higher doses. Exposure periods had an immediate significant effect on larvae and adult mortalities 1 and 6 h after the insects were introduced. At a 15% concentration, EO with a 0 h pre-exposure period had significantly higher adult and larva mortalities after 6 h than those of 0.5, 1 and 2 h pre-exposure. Regardless of oil pre-exposure, insects were repelled, especially in test periods between 12 and 30 min. Therefore, grapefruit EO could be formulated for increased stability when an immediate impact is needed.
Keywords: exposure periods; grapefruit peel; mortality; pest control; toxicity.
Response of Sweet Corn Varieties to Plant Density and Tiller Removal: Preliminary Studies
Răzvan Huțuliac, Cristina Precupeanu, Ionela Cătălina Vasilachi, Alexandru Cojocaru, Mihaela Roșca, Vasile Stoleru
ABSTRACT. The sweet corn breeding strategy aims to enhance and sustain a consistent yield over time. The interaction between density and tiller removal is currently being discussed among sweet corn farmers, as well as scientists working in sweet corn. Considering the studies carried out to date and the research directions suggested by them, the present study aimed to determine the impact of plant density, variety, tiller removal and their interactions on the yield and ear prolificacy/weight of sweet corn under the climatic conditions of northeast Romania. The experiments were focused on 3 sweet corn varieties (Deliciosul de Bacău, Deliciul verii and Royalty F1), sown at 3 densities (50k, 60k and 70k plants/ha) and considering 2 variants for tiller removal (tiller removal / without tiller removal). The highest yield of sweet corn was recorded in the Royalty F1 hybrid in the plots in which the tillers were removed (24.27 t/ha). The Royalty F1 hybrid also had the heaviest ears (313.33 g) in plots with tiller removal and 60k plants/ha. Overall, the most favourable plant density in terms of yield was 60k plants/ha. The experimental results showed that in the climatic conditions of northeast Romania, tiller removal led to increased yield, especially in the Deliciosul de Bacău hybrid. Tiller removal in this hybrid resulted in a significant increase in yield (from 18.41 to 23.08 t/ha).
Keywords: plant density; sweet corn variety; tillers impact; yield.
A New Natural Food Dye: Microencapsulated Cornelian Cherry Bioactive Compounds
Iuliana-Maria Enache, Liliana Lucescu Ciurlă, Nicoleta Stănciuc, Antoanela Patraș, Camelia Vizireanu, Liviu Mihai Irimia
ABSTRACT. Cornus mas (CM) is one of the four edible fruits of the Cornus genus, a rich source of biologically active compounds (BACs) such as vitamins (like vitamin C), carotenoids, iridoids, and phenolics (phenolic acids, anthocyanins, and other flavonoids). This study aimed to analyse the improvement of the stability of CM anthocyanins by microencapsulation, in order to propose a new natural food dye. Microencapsulation using a mixture of whey protein isolate (WPI) and chitosan (CH) as wall materials has been applied to protect anthocyanins against external factors (e.g., light, temperature, storage, etc.). Two experimental variants of microencapsulated powders, WPI:CH = 1:1 (CH1) and WPI:CH = 1:2 (CH2), were realised by varying the wall materials ratio. The cornelian cherry fruit concentrated extract was evaluated for its phytochemical, colourimetric, and antioxidant capacities. Due to the excellent anthocyanin encapsulation effectiveness (74.29 – 88.71%), the wall materials utilised for both powders can be considered effective choices to safeguard the anthocyanins. All tests performed on the microencapsulated powders demonstrated that both suggested experimental forms can serve as a healthy substitute for artificial food additives. The incorporation of cornelian cherry fruit extract and microencapsulated powders into a food matrix (jelly candies) allowed examination of their effectiveness. The colour analysis rigorously characterised all the colour parameters related to red nuances (due to anthocyanins content, such as cyanidin-3-glucoside) and yellow nuances (associated with carotenoids content).
Keywords: chitosan; jelly candies; natural pigments; phenolic compounds; whey protein isolate.
Effect of Okoro [(Albizia zygia (J.F.) Macbr)] Leafy Biomass and NPK Fertiliser on the Growth and Yield of Sweet Pepper (Capsicum annum L.)
Adams Latif Mohammed
ABSTRACT. Agricultural productivity and sustainable crop management are pivotal aspects of global food security and economic stability. The quest for efficient and environmentally friendly practices in crop production has led to a surge in research exploring the utilisation of organic materials alongside conventional fertilisers to enhance crop growth and yield. An experiment was conducted over a growing period of three months, starting in August 2023 at the research field of Kwame Nkrumah University of Science and Technology, Kumasi-Ghana, to investigate the effects of Albizia zygia leafy biomass in combination with NPK fertiliser on the growth and yield of sweet pepper (Capsicum annum L.). A randomised complete block design was employed with four treatment groups replicated four times, including various combinations of A. zygia leafy biomass and NPK fertiliser, alongside control groups of sole fertiliser application and untreated plots (T1). The treatments were as follows: T1 = No biomass, no NPK (control); T2 = 0.5348 kg of A. zygia leafy biomass per 4.86 m2 (1100.5 kg/ha of A. zygia leafy biomass); T3 = 0.2463 kg of NPK per 4.86 m2 (506.7 kg/ha of NPK); and T4 = 0.2674 kg of A. zygia leafy biomass per 4.86 m2 + 0.1231 kg of NPK (550.3 kg/ha of A. zygia leafy biomass + 253.4 kg/ha of NPK). The assessed parameters were plant height, number of leaves, number of branches, fruit yield and fresh fruit weight. Significant variations (p ˂ 0.05) were observed in the growth parameters (plant height, number of leaves, number of branches) and yield attributes (fruit yield and fresh fruit weight) of sweet pepper among the treatments. The combined application of A. zygia leafy biomass and NPK fertiliser (T4) exhibited increased effects on plant height, number of leaves, number of branches, fruit yield and fresh fruit weight compared to individual treatments and control groups (p ˂ 0.05). Notably, the treatment combining A. zygia leafy biomass and NPK fertiliser (T4) demonstrated a substantial increase in plant height (44.58 cm), a significant improvement in the number of leaves (42.98) and a remarkable enhancement in the number of branches (11.33) compared to the sole applications and the control group. Furthermore, the combined treatment significantly increased (p ˂ 0.05) the yield parameters, including fruit yield and fresh fruit weight per hectare, showcasing an increase of 8796/ha and 23.91 t/ha, respectively, compared to sole fertiliser application and the control. The findings from this study demonstrated that the incorporation of A. zygia leafy biomass, either solely or in combination with NPK fertiliser, positively impacted the growth and yield of sweet pepper plants. These results highlight the potential of A. zygia leafy biomass as a valuable organic amendment for sustainable and enhanced crop production, offering promising implications for agricultural practices aimed at improving yield and plant growth.
Keywords: Albizia zygia; Capsicum annum (L.); NPK fertiliser; organic amendment; sweet pepper.
Characterisation of Macro-Plastic Waste Along The Philippines Longest Coastal Boulevard: Basis for Solid Waste Management and Policy Formulation
Lucy O. Elep Jr., Maria Cristina C. Azuelo
ABSTRACT. This study focused on the characterisation of macro-plastic waste in terms of types and quantities along the Philippines’ longest coastal boulevard, considering density and cleanliness as a basis for solid waste management and policy formulation. Macro-plastic identification and counting were conducted in four (4) municipalities and eight (8) barangays using the standing stock survey method. There were six (6) types of macro-plastic waste, with the highest quantity consisting of beverage (37.2%) and food packaging (30.5%), followed by fishing gear (17.0%), plastic utensils (9.2%), toiletries (4.8%) and household wastes (1.4%). The computed overall density from a total of 3,978 macro-plastic waste items over 12,000 m2 of beach area sampled was 0.3 (CM), meaning there were 0.3 litter items of plastic per m2 throughout the whole boulevard. The computed overall beach cleanliness was 6.6, which means that the cleanliness status is moderate. Overall, most macro-plastic waste is generated by locals or visitors. In conclusion, a policy regulating the use, littering, and carrying of plastic along boulevards is recommended to prevent plastic pollution considering the province’s growing ecotourism and the future expansion of the coastal boulevard.
Keywords: coastal road; ecotourism; social plastic.
Cover Story
Jellyfish (JF) are essential to marine ecosystems. However, JF that develop rapidly can have negative effects. On 3–4 August, 2022, a significant JF (Lobonemoides robustus Stiasny, 1920) bloom was observed along the Cox’s Bazar coast in Bangladesh (from Najdirartek to Sabrang). The goal of the current investigation was to identify the fatty acids (FAs) and amino acids (AAs) of L. robustus. The AAs were determined using liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis, while the FAs were determined using a gas chromatographic system with a flame ionisation detector. The most prevalent AA was glycine. The most common FA was linoleic acid (C18:3) (0.43%), followed by myristic acid (0.12%), cis-9-oleic acid (0.18%), gamma-linolenic acid (0.24%), and heptadecanoic acid (0.29%). Based on its high AA and FA contents, L. robustus is a great candidate for the potentially sustainable manufacture of nutraceutical, cosmeceutical, and biomedical natural products to improve health and well-being. In addition, the edible L. robustus could be exported to other countries for use as the bloom species, thus playing a major role in achieving a blue economy (see page 107).
Extraction and Characterisation of Natural Dye From Orange Peel for Textile Applications
Usman Habu Taura, Muhammad Abbagoni Abubakar, Abdulhalim Musa Abubakar, Mohammed Umar Kurgiya
ABSTRACT. Awareness of the need to protect the environment and people’s health has led to an intensification of concerns for obtaining sustainable products and processes. Toxic waste created during the production and use of synthetic dyes has an impact on both human and environmental health. As a result, natural dyes are more secure and safer than synthetic dyes. This study is significant because it has the potential to help develop sustainable and environmentally friendly textile dyeing techniques. In this study, a natural dye was successfully extracted from orange peel (Citrus recticulata Rutaceae) which was applied on textile fibre and was found to be partly effective for eco-friendly dyeing applications. The extracted dye does not have a good wash fastness, which is the ability of the fabric to retain its colour after washing. This weakness is demonstrated by carrying out a Fourier transform infrared (FTIR) analysis where the potential binding mechanisms between the dye and the textile fibres was revealed. Different functional groups can interact with the fibre’s functional groups, affecting the dye’s affinity for the fabric and its overall colour fastness properties. Findings show that dyes from orange peels showed promise in this study, but need to be improved further. Therefore, the study suggests that further research is needed to optimize the dyeing process and improve the fabric’s resistance to washing and other environmental factors.
Keywords: mordant; natural dye; orange peel; synthetic dye; textile industry.
Correction: Usman Habu TAURA’s affiliation has been changed from Department of Chemical Engineering, University of Maiduguri, P. M.B 1069 Maiduguri, Borno State, Nigeria in Oil and Gas Research Centre, Sultan Qaboos University, Muscat, Oman, 123, according to supporting document Journal of Applied Life Sciences and Environment_affiliation correction justification
Assessment of Sensitivity to Desertification in the Oum Er-Rbia Watershed (Upstream of Ouled Sidi Driss) Using The Medalus Approach
Souad Ourabit, Abderrahim Ettaqy, Mohamed El Ghachi
ABSTRACT. The issue of desertification is a pressing concern for many vulnerable regions, with consequences that extend far beyond their borders. It is a silent force that not only contributes to global climate change by releasing stored carbon from vegetation and soil but also compounds the ongoing environmental challenges we face on a global scale. In Morocco, the effects of these climate changes are already noticeable, particularly in terms of water scarcity due to reduced rainfall and rising temperatures. This, in turn, leads to soil drying and an increased risk of degradation. The Oum Er-Rbia watershed (upstream of Ouled Sidi Driss) is one of the basins affected by this issue, covering an area of 11,152 km² and spanning three topographic units (the Middle Atlas Mountains, the Phosphate Plateau and the Tadla Plain). This topographic diversity causes regional variation in the sensitivity to desertification. Therefore, this study aims to evaluate the sensitivity to desertification in this watershed by using the MEDALUS model (Mediterranean desertification and land use), which calculates the Desertification Sensitivity Index (DSI) through the composition of four indices: the Soil Quality Index (SQI), the Vegetation Quality Index (VQI), the Climate Quality Index (CQI) and the Land Use Quality Index (LUQI). The results show that the critical and highly sensitive zones represent 44% and are located at the northeast and west ends of the watershed. By contrast, the unaffected zones represent 12% of the total area of the watershed and correspond to the upstream area, where the climate, vegetation and land use systems are of good quality, providing effective protection for the land. To reduce the impact of this phenomenon, we have proposed some potentially impactful development actions based on land use and the results obtained.
Keywords: climate change; desertification sensitivity; drought; MEDALUS; Oum Er-Rbia watershed.
Growth Performance, Nitrogen Utilization and Economics of Red Sokoto Bucks Fed Saccharomyces Cerevisiae-Treated Groundnut Haulm and Dried Cassava Peel-Based Diets
Ahmad Rufai Musa, Olayinka John Makinde, Aminu Maidala, Ahmed Bishir, Kakudi Ismail Abubakar, Kosoro Ahmad Mua’zu, Ismaila Habiba Abdullahi, Yakubu Zakari Idrissa, Charles Hannatu, Muhammad Hamisu Zango
ABSTRACT. This study investigated the growth performance, nitrogen balance, and economics of production of Red Sokoto bucks (RSBs) fed Saccharomyces cerevisiae-treated, biodegradable groundnut haulms (SCGH) and dried cassava peels (DCP). Four dietary treatments were formulated including a control diet (T1; 0% SCGH:DCP) and DCP in combination with SCGH at the different proportions of 275:695 (T2), 375:595 (T3), and 475:495 (T4) g/kg of dry matter (DM). A combination of 5 g and 50 g of yeast as a probiotic and molasses, respectively, were added to 1 kg of GH after been dissolved in 1 L of water. Twelve healthy RSBs weighing 9.5 ± 0.40 kg of body weight (BW) and aged 7 to 8 months were exposed to four treatments. This was replicated three times in a completely randomized design (CRD). The experiment lasted for 90 days. The average final weight, average daily weight gain (gram/day), nitrogen intake, and nitrogen balance were significantly influenced by the inclusion of DCP and SCGH in the diets. There was no significant difference (p > 0.05) between the ADG of T1 (58.20 g/d) and the T4 (58.89 g/d) groups. Feed conversion ratio and feed-cost saving ($630.80) increased with the inclusion of DCP and SCGH in the goats’ diets. In conclusion, T4 (SCGH: 475g, DCP: 495g) had a better chemical composition, ADG, nitrogen balance, and feed-cost saving; therefore, SCGH are recommended and are readily available agricultural wastes that can be fed to RSBs to improve performance and nitrogen utilization in a cost-effective way, especially during dry season.
Keywords: bucks; cassava peels; nitrogen balance; probiotics; yeast.
First Report of The Amino Acid And Fatty Acid Composition of Jellyfish (Lobonemoides Robustus Stiasny, 1920) Collected During Jellyfish Bloom Along the Cox’s Bazar Coast, Bangladesh
Tarikul Islam, Simul Bhuyan, Mala Khan, Mrityunjoy Kunda, Sumi Akter, Nayan Kumer Kundu
ABSTRACT. Jellyfish (JF) are essential to marine ecosystems. However, JF that increases rapidly can have negative effects. On 3-4 August 2022, a significant JF (Lobonemoides robustus Stiasny, 1920) bloom was observed along Cox’s Bazar coast (from Najdirartek to Sabrang) in Bangladesh. The goal of the current investigation was to identify the fatty acids (FAs) and amino acids (AAs) of L. robustus. The AAs were determined using liquid chromatography–tandem mass spectrometry (LC-MS/MS) analysis, while the FAs were determined using a gas chromatographic system with a flame ionisation detector. The most prevalent AA was glycine. The most common FA was linoleic acid (C18:3) (0.43%), followed by myristic acid (0.12%), cis-9-oleic acid (0.18%), gamma-linolenic acid (0.24%), and heptadecanoic acid (0.29%). Based on its AA and FA contents, L. robustus can be a great candidate for the potentially sustainable manufacture of nutraceutical, cosmeceutical, and biomedical natural products to improve health and well-being. In addition, the edible L. robustus could be exported to other countries, thus way it can play a major role in achieving a blue economy.
Keywords: amino acids; bloom; blue economy; fatty acids; jellyfish.
Foliar Zinc and Soil Applied Molybdenum Optimize Yield and Dry Matter Partitioning of Lentil
Shams Ur Rehman, Uzair Ahmed, Ayesha Zahoor, Zia Ur Rahman, Ikram Ullah, Salman Khan, Rizwan Ali, Osama Muhammad Jafar, Muhammad Taimoor, Aamir Sohail, Sajid Ali
ABSTRACT. A field experiment on the effect of zinc and molybdenum application on the yield of lentil was carried out at The University of Agriculture Peshawar during the Rabi season of 2021. The experiment was carried out in a randomised complete block design with three replications. Four levels of molybdenum (0, 1, 2 and 3 kg ha−1) and 0.5% zinc foliar spray at different intervals of lentil crop (control, 50 days after sowing, 100 days after sowing, 50 days after sowing + 100 days after sowing) were tested in the experiment. The results revealed that 3 kg ha−1 of molybdenum enhanced plant height (55 cm), nodules plant−1 (25), branches plant−1 (7), days to flowering (107) and maturity (159), dry matter of leaves at the flowering stage (64 g m−2), dry matter of leaves at the maturity stage (113 g m−2), dry matter of branches at the physiological maturity stage (304 g m−2), dry matter of pods at the maturity stage (439 g m−2), pods plant−1 (92), seeds pod−1 (2.0), 1000-seed weight (31 g), biological yield (3207 kg ha−1), and seed yield (1002 kg ha−1). Zinc foliar spray (0.5%) at 50 days after sowing + 100 days after sowing of lentil crop improved the dry matter of leaves at the flowering stage (62 g m−2), days to first flowering (109), days to physiological maturity (157), dry matter of leaves at the physiological maturity stage (111 g m−2), dry matter of pods at the physiological maturity stage (435 g m−2), pods plant−1 (91), 1000 seed weight (32 g), biological yield (3236 kg ha−1) and seed yield (1026 kg ha−1). Lastly, scatterplots revealed a significant correlation of grain and biological yield with their components. It is concluded that molybdenum application at a rate of 3 kg ha−1 and 0.5% zinc foliar spray at 50 days after sowing + 100 days after sowing of the lentil crop increased seed yield and is thus recommended for the Peshawar region.
Keywords: correlation; cropping system; fertilisation; Lens culinaris L.
Estimation of Heritability, Genotypic Variability and Correlations Analysis for Yield and Yield Attributing Traits Among Bread Wheat (Triticum aestivum L.) Genotypes
Mansoor Javed, Akbar Ali, Muhammad Kashif, Muhammad Ali, Saif Ullah, Ayesha Alam
ABSTRACT. To find out genetic variability, heritability, and trait association among yield and yield-related traits among bread wheat genotypes, an alpha lattice design was used in triplicate manner where 50 wheat genotypes were evaluated at the University of Agriculture Peshawar during rabbi growing season 2021-22 along with a regional check genotype. Data were taken on ten parameters. All the genotypes showed significant variation among them, signifying the possibility of enhancing genetic improvement through breeding programs. Highly significant differences were found in days to heading, days to maturity, plant height, spike length, grain filling duration, number of grains per spike, biological yield and grain yield these traits indicating diversity in yield potential. Moderate to low heritability values were noted for most of the traits. The study exhibits positive correlations for plant height with grain yield, spike length with biological yield and harvest index with grain yield. On the basis of high heritability and positive correlation of grain yield with other traits, it is recommended that G-41, G-3, G-12, G-37, G-34 and G-14 genotypes which have the potential to be incorporate in further breeding programs.
Keywords: correlation; crop improvement; heritability; quantitative genetics; variability; wheat breeding; wheat genotypes.
Preliminary Studies on Some Morpho-Structural and Biochemical Characterisation of Some Genotypes of Vitis vinifera L. Cultivated in Northeast Romania
Liliana Rotaru, Vasile Răzvan Filimon, Roxana Mihaela Filimon, Mihai Mustea, Roberto Renato Bernardis, Lucia Cintia Colibaba
ABSTRACT. The monitoring of new grapevine varieties with superior agrobiological and technological characteristics, in relation to the evolution of the climatic factors, represents an important and continuous objective of the worldwide viticultural research and breeding programs. Observations and determinations of the current study were performed on 11 new table grapes varieties created in Romania, growing in the Ampelographic collection of the University of Life Sciences Iasi, north-eastern area of Romania. The grapevine varieties were evaluated under the morpho-structural aspect, regarding the leaf area, the average weight of the grapes, the number and weight of the berries, rachis weight, the number and weight of the seeds, the weight of the skin and pulp, calculating the main technological indices. From a biochemical point of view, the content of photosynthetic pigments in leaves, the concentration of soluble dry solids and titratable acidity of the grapes were determined in the climatic condition of the Copou – Iasi vineyard. Therefore, the monitoring of genetic resources provides useful data for grape producers and researchers regarding the integration of new table grape varieties into the viticultural ecosystems, evaluating their yield and quality in correlation with the evolution and influence of the climatic factors.
Keywords: agrobiological and technological value; autochthonous grapevine variety; chemical composition; genetic resources; physico-structural characterization.
Aspects of the Contribution of the Leader Approach to Rural Development in Romania. Case Study: North-East Development Region
Carmen-Olguța Brezuleanu, Mădălina-Maria Brezuleanu, Roxana Mihalache, Irina Susanu, Diana Elena Creangă, Elena Ungureanu
ABSTRACT. Rural development is the second pillar of the Common Agricultural Policy of the European Union (EU), with the role of helping the rural areas of the EU and implicitly Romania to address the economic, environmental, and social challenges they face. The purpose of the research included in this paper is to demonstrate the contribution of the LEADER approach to rural development in Romania, with an emphasis on the North-East Development Region. At the same time, the aim was to highlight the innovative nature of this approach: what it means, how it can be applied, and how it was applied. The data studied through the analysis carried out show that the Romanian territory and, implicitly, the North-East Development Region is poor, fragmented, depopulated, or in the process of depopulating, with few opportunities for young people. The innovative character of the LEADER Programme in Romania and implicitly the North-East Development Region results from the degree of novelty that an investment financed through it brings to the targeted territory, without being limiting and without necessarily presupposing a technological innovation, because the innovation must be evaluated relative to the local situation. The main instrument through which the principles of the LEADER approach can be implemented is the Local Action Group. It is the main driving force behind the activities to be carried out in the territory and which will lead to their implementation. The Local Action Groups set up in the North-East Region provide a common communication framework for local communities to develop and implement Local Development Strategies by initiating, developing and financing projects at local level. They contribute to the unity of local communities and their participation in local development. LEADER approach has brought and how its innovative character is highlighted. The questions that informed its development were: Is this concept considered as a model for sustainable rural development in Romania and the N-E Region? Is LEADER a truly innovative approach. In order to achieve the proposed goal, a multi-step working procedure was developed to allow the collection of target data and additional data derived from the initial target data. Thus, the working procedure was structured in the following steps: problem identification and conceptualization, literature review, document structuring, strategy selection, operational planning, data calculation, and interpretation. Both qualitative and quantitative methods were used in this work. Thus, from a quantitative point of view, the following research methods were considered relevant for obtaining data: administrative data analysis. As a qualitative method, a bibliometric analysis was carried out, i.e., the literature on sustainable rural development through the use of support measures was analysed by means of the VOSviewer programme, using the Web of Science collections database. Without the implementation of the LEADER Programme in Romania and implicitly in the North-East Development Region, rural areas may be deprived of funding that determines the improvement of conditions in that area, but efforts in the field of implementing sustainable rural development measures must be continued so that the effect of this funding is really visible. Thus, the results of the research carried out in the North-East Development Region of Romania add additional value to the information published in previous studies through proposals for rural reform and concrete examples of innovative projects implemented there.
Keywords: LEADER; local action group; North-East Development Region; Romania; rural development.
Raw Bovine Milk as a Reservoir of Multi-Drug Resistant, Beta-Lactamase-Producing Klebsiella
Achintya Mahanti, Siddhartha Narayan Joardar, Samiran Bandyopadhyay, Jaydeep Banerjee, Sarbaswarup Ghosh, Tapan Kumar Dutta, Indranil Samanta
ABSTRACT. The transmission of zoonotic bacteria through consumption of raw milk is complicated by the dissemination of antimicrobial-resistant bacteria. The present study was conducted to detect the occurrence of antimicrobial-resistant bacteria (ESBL-/AmpC-producing Klebsiella spp.) in cow’s milk originating from healthy or infected (mastitis) cattle in India. In total, 450 milk samples were collected from apparently healthy cattle and cattle suffering from clinical or sub-clinical mastitis. Out of 455 Klebsiella spp., 67 (14.73%) isolates were found to be ESBL producers in the double-disc diffusion test. The occurrence of ESBL-producing Klebsiella spp. was significantly (p < 0.05) higher in milk samples collected from cattle suffering with mastitis than in healthy cattle. Among the ESBL-producing Klebsiella spp., 56 (83.6%) isolates were also detected that produced AmpC β-lactamases. All the ESBL and AmpC-producing Klebsiella spp. possessed blaCTX-M (100%) and blaAmpC (100%), respectively. The present study revealed a higher occurrence of class 1 integron in ESBL-producing Klebsiella spp. isolates. All ESBL-producing- Klebsiella spp. isolates were multi-drug resistant. The ciprofloxacin- and/or levofloxacin-resistant Klebsiella spp. isolates possessed the quinolone resistance gene (qnrS). The co-trimoxazole-resistant isolates possessed the sul1 and sul2 genes. Phylogenetic analysis of the studied isolates revealed that strains isolated from the same location had a clonal relationship. The study increases consumer awareness of the need to avoid raw milk consumption to prevent the spread of antimicrobial resistance in the community.
Keywords: antimicrobial resistance; ESBL; Klebsiella; MIC; raw milk.
Cover story
Watermelon production is affected by root-knot nematode (RKN), as all varieties of the crop are susceptible. Studies have been conducted to identify readily available and environmentally safe nematicides for the management of RKN. Aqueous and powdered extracts of Chromolaena odorata (L) King and Robinson and Ficus mucuso Welw. ex Ficalho were applied alone and in combination. All data collected on growth, yield and nematode populations were subjected to analysis of variance, and treatments were compared using Duncan’s multiple range test at a 5% level of significance. Among the treatments, the combination of C. odorata and F. mucuso powders was the most effective, with higher growth and yield performance. The experiment showed that aqueous and powdered extracts of C. odorata and F. mucuso were effective in managing RKN in the field (see page 527)
