Online: ISSN 2784 – 0360 | ISSN – L 2784 – 0360 | Print: ISSN 2784 – 0379 ISSN-L 2784 – 0360

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Jităreanu Carmenica Doina

Comparative Analysis of The Influence of Different Biostimulators on the Germination and Sprouting Behaviour of Four Wheat Varieties

Mar 23, 2023 |

Alina Elena Marta, Cristina Slabu, Mihaela Covașă, Iuliana Motrescu, Carmenica Doina Jităreanu

ABSTRACT. Wheat is an important cereal around the world and is a nutrient source in people’s diets. In this work, we focused on the potential of some biostimulators on four wheat varieties – Dropia, Glosa, Gruia, and Pegasus, with a focus on the effects during the first developing stages from germination to the appearance of the second leaf. Our results indicated that germination and some biochemical traits can be strongly influenced by biostimulants, with the effects also depending on the genetic background of the variety. Some treatments proved beneficial for germination, and also growth as found by the biometric measurements, while others inhibited both traits. Some of the biostimulants increased the concentration of the photosynthetic compounds, thus being recommended for use during all stages of wheat development. Dropia, Glosa, and Gruia reacted positively in most tests, their germination and plant and root development were stimulated by Super fifty, Atonik, Asfac, and Cropmax. Thus, we recommend the use of these biostimulants in the first development stages. The concentrations of photosynthetic pigments increased after treatment with Asfac, Atonik, and Cropmax. The treatment showing the most inhibitory effects was Lebosol.

Keywords: wheat; biostimulator; wheat sprouts.

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Influence of Foliar Fertilisers on Biochemical and Physiological Properties in Nepeta Racemosa LAM.

Feb 23, 2022 |

Constantin Lungoci, Carmen Doina Jităreanu, Carmen Simona Ghițău, Teodor Robu

ABSTRACT. The purpose of this study was to evaluate some elements of physiology and biochemistry in Nepeta racemosa Lam. in the pedo-climatic conditions of the Moldavian forest steppe. The parameters were quantified under conditions of differentiated fertilisation with three foliar products (KSC 1, KSC 2, and Corona K). Biochemical analysis by GC/MS revealed the highest concentration of the following components: nepetallic acid (Untreat – 54.33%, KSC 1 – 55.50%, KSC 2 – 56.90%, Corona K – 53.77), eucalyptol (Untreat . 9.87%, KSC 1 – 10.70%, KSC 2- 12.80%, Corona K – 10.78%), 3-hydroxy-(4S, 4aS, 7S, 7aS)-dihydronepetalactone (Untreat – 5.87%, KSC 1 – 5.37%, KSC 2 – 4.87, Corona K – 3.67%), and (4aS, 7S, 7aS)-trans, cis-nepetalactone (Untreat – 4.30%, KSC 1 – 5.00%, KSC 2 – 4.20%, Corona K – 5.23%). Polyphenols were present in large quantities in the Corona K variant, with 33.1 mg GA/g dw at the first harvest and 27.88 mg GA/g dw at the second harvest. The highest concentration of flavonoids was identified in the Corona K variant (26.34 mg QE/g dw – first harvest and 56.77 mg QE/g dw – second harvest). The antioxidant activity was between 14.76 and 38.54% at the first harvest, and from 12.18 to 22.95% at the second harvest. Chlorophyll pigments ranged from 13.12-17.68 μg/ml-1 for chlorophyll a, 5.43-9.66 μg/ml-1 for chlorophyll b, and 2.07-3.34 μg/ml-1 for carotenoids. The highest grass production per m2 was achieved in the version fertilised with KSC 1 for both crops, with 986.66 g/m2 for the first harvest and 861.33 g/m2 for the second. Iridoids are a class of substances that are commonly used for pesticide role. The study aims to highlight these substances and determine whether differentiated fertilization influences. The presented data confirm the results in the literature for polyphenols and flavonoids, but also present chemotypic novelties in terms of chemical composition and represent a direction to follow for future research.

Keywords: nutrients; flavonoids; nepetalic acid; polyphenols; chlorophyll; DPPH.

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The relationship between chlorophyll content and antioxidant activity of Abies Alba and Nepeta Pannonica extracts according to phenophase and harvesting area

Oct 18, 2019 |

S. Buhăianu, Doina Carmen Jităreanu

ABSTRACT. Abies and Nepeta species are well known for their traditional use in traditional medicine from Bukovina, Romania, and other parts from the world. However, too few studies present the connection between phenophase, chloro-phyll content and antioxidant activity of extracts obtained from medicinal plants. Phenophases, like stages from vital cycle of plants, involve seasonal and evolu-tionary changes, including chlorophyll content of the leaves. With these changes, there are also changes in the oxidative activity of the extracts obtained from the studied plants. The chlorophyll was extracted with acetone, being quanti-tatively measured using the spectro-photometer. Antioxidant activity was determined by the DPPH method. This method is one from the most popular ways to measure the antioxidant capacity of a substance. The studied species are Abies alba and Nepeta pannonica. Plants were harvested from different locations of Bukovina, from areas of Câmpulung Moldovenesc and Cacica localities, Suceava county, Romania. Chosen phenophases were growing and flowering. The DPPH method implies making some extracts with organic solvents from collected plants, in this case being ace-tone. Using 2,2-diphenyl-1-picrylhydrazil reactive, there was determined the antioxidant capacity of mentioned extracts. There were observed variations of this depending of phenophase and collecting areas, being closely related to total chlorophyll content. The both species have behaved differently and obtained results can be used to determine the optimal harvest moments of these plants.

Keywords: growing; flowering; medicinal plants; spectrophotometer

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Variations of chlorophyll content at Abies Alba and Nepeta Pannonica species according to phenophase and harvesting area

Oct 10, 2019 |

S. Buhăianu, Doina Carmen Jităreanu

ABSTRACT. Chlorophylls from plants are photosynthetic pigments. Their quantity offers valuable informations about photosynthetic activity, growing and developing of plants. Photosynthetic pigments decrease quantitatively during senescence process or in stress conditions. The present study has been realized in laboratory conditions with material harvested from spontaneous flora. The purpose of this research was the investigation of variations of chlorophyll content from samples of biological material collected from Nepeta pannonica L. and Abies alba Mill. plants, from Câmpulung Moldovenesc and Cacica areas, Suceava county, Romania. The targeted phenophases were growth and flowering. There were realized acetonic extracts from samples for spectropho-tometric determinations. Obtained data were processed to estabilish chlorophyll a and b content. There were observed that at Abies alba species, from both locations, the chlorophyll a content grew during flowering phenophase, while the chlorophyll b content had little variations. At Nepeta pannonica species, the chlorophyll a and b content decreased visibly during the flowering, due to stress. Leaves of plants from this species presen-ted a intense green color in the growing phenophase, while during flowering phe-nophase they had a purple or yellow colo-ration. Obtained results revealed a different dynamics of chlorophyll content at studied species.

Keywords: photosynthesis, plants, pigments, flowering.

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© by the authors; licensee Journal of Applied Life Sciences and Environment, Iasi, Romania. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)