Online: ISSN 2784 – 0360 | ISSN – L 2784 – 0360 | Print: ISSN 2784 – 0379 ISSN-L 2784 – 0360

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Ansari O.

The Effect of Priming on Germination Characteristics of Barley Seeds under Drought Stress Conditions

Jun 5, 2020 |

S.A. Tabatabaei, O. Ansari

ABSTRACT. Seed priming methods have been used to increase germination and seedling establishment under different abiotic stress conditions. Seed priming was used in barley to increase seed germination and tolerance on stress exposure. Barley seeds were treated with various priming agents for different time and temperatures. The effect of priming was assessed on germination characteristics on subsequent exposure to drought (PEG-12 bar) stress for 7 days. Seed priming treatments significantly (p≤ 0.01) affected germination percentage (GP), normality seedling percentage (NSP), germination Index (GI), germination uniformity (GU), means time to germination (MTG), coefficient of velocity of germination (CVG), seedling vigor index (SVI) and coefficient of allometry (AC). Seed priming with gibberelic acid (GA), salicylic acid (SA), ascorbic acid (ASc), hydropriming (HP), osmopriming (OP) and combined treatments (CT) significantly (p≤ 0.01) increased germination characteristics, compared to the unprimed. Seed priming with concentrations 25, 50 ppm of GA for 15 h at 10°C, 25 ppm of SA for 12 h at 10°C, 50 ppm of ASc for 12 h at 15°C, treatment 16 h hydropriming at 10°C and -15 bar PEG for 24 h for osmopriming maybe considered as optimal treatment for priming of barley seeds in drought stress conditions. In some cases, combined treatments are better than the separate treatments. Seed priming with PEG (potential -l5 bar PEG for 24 h at 10°C) was more effective in drought stress than the other treatments.

Keywords: seed priming; Hordeum vulgare L.; drought stress.

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Quantification of germination response of millet (Panicum Miliaceum L.) seeds to water potential and priming using hydrotime model

Oct 18, 2019 |

S.A. Tabatabaei, N. Bayatian, S. Nikoumaram, O. Ansari

ABSTRACT. Seed germination is a com-plex biological process that is influenced by different environmental physical factors including temperature, water potential, salinity, pH and light, as well as intrinsic genetic factors. In such environments, the water needed for germination is available for only a short time, and consequently, successful crop establishment depends not only on rapid and uniform germination of the seedlot, but also on its ability to germinate under low water availability. All of these attributes can be analyzed through the hydrotime model (HT). Millet (Panicum miliaceum L.) is cultivated in arid and semi-arid regions of Iran. Therefore, in this study, using the hydrotime modeling approach, germination response of millet to priming (water and gibberellin 50 ppm at 15°C for 24 h) and water potential (0, -0.3, -0.6, -0.9, and -1.2 Mpa) was studied. Hydrotime (HT) model were fitted to cumulative germination of seeds and recorded in germination tests carried out at different water potentials (0, -0.3, -0.6, -0.9 and -1.2 MPa) and priming treatments (control, hydropriming and hormone priming). Results showed that, germination of millet decreased significantly with reduction of osmotic potential. Results indicated that the hydro-time constant (θH) for control, hydro-priming and hormone priming were 0.89, 0.79 and 0.67 MPa d, the water potential (Ψb(50)) for control, hydropriming and hormone priming were -0.89, -0.94 and -1.11 MPa, respectively. Results indicated that the use of hydrotime model in germination prediction could be useful to provide more accurate estimates for the timing of sowing and management of millet.

Keywords: base temperature; base water potential; germination prediction; hydrotime; Panicum miliaceum L.

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© by the authors; licensee Journal of Applied Life Sciences and Environment, Iasi, Romania. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)